Chromatograms, MS and MS/MS spectra obtained by LC-MS/MS (Q-TOF) of peptides from UPIII identified after Western blotting followed by on-membrane digestion.

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Chromatograms, MS and MS/MS spectra obtained by LC-MS/MS (Q-TOF) of peptides from UPIII identified after Western blotting followed by on-membrane digestion (NC-free method) but not by conventional in-gel digestion.a, e, extracted ion chromatogram (m/z 747 and 1014, respectively) after in-gel digestion. b, f, extracted ion chromatogram (m/z 747 and 1014, respectively) after on-membrane digestion. c, g, MS spectra after on-membrane digestion. d, h, MS/MS spectra after on-membrane digestion. Chromatograms, MS and MS/MS spectra obtained by LC-MS/MS (Q-TOF) of peptides from UPIII identified after Western blotting followed by on-membrane digestion (NC-free method) but not by conventional in-gel digestion.a, e, extracted ion chromatogram (m/z 747 and 1014, respectively) after in-gel digestion. b, f, extracted ion chromatogram (m/z 747 and 1014, respectively) after on-membrane digestion. c, g, MS spectra after on-membrane digestion. d, h, MS/MS spectra after on-membrane digestion. Jose L. Luque-Garcia et al. Mol Cell Proteomics 2008;7:308-314 © 2008 The American Society for Biochemistry and Molecular Biology