Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. Analytical.

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Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. The Q-OT-qIT (panel A, red) achieves identification of up to 19 peptides per second as compared with 10 with the qIT-OT system (A, black). Peak depth is likewise considerably higher on account of the faster MS2 scanning rate of the Q-OT-qIT system (B). C, plots the pace of unique yeast peptide identifications for the three instruments. For the one hour analysis, the Q-OT-qIT posts almost twice as many unique peptide identifications as compared with the qIT-OT. Similar data, except for unique proteins, is shown in D. Alexander S. Hebert et al. Mol Cell Proteomics 2014;13:339-347 © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.