a b NHS/RA Serum IL-17FF pg/mL % Recovery n=5 SD

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a b NHS/RA Serum IL-17FF pg/mL % Recovery n=5 SD Normal CSF IL-17FF pg/mL 0.1 99.8 20.86 6.26 114.2 4.02 0.2 107.2 10.96 0.4 94.4 5.86 0.3 105.8 8.76 1 97.8 5.17 10 104 4.95 NHS/RA Serum IL-17AA pg/mL Normal CSF IL-17AA pg/mL 120.2 9.68 118 5.61 105.4 8.17 95.4 7.54 88 7.68 102 2.35 Table S2. Average percent recovery of recombinant IL-17AA and IL-17FF in analyte-depleted serum (a) and CSF (b). (IL-17AA: 4 NHS and 1 RA. IL-17FF 3 NHS and 2 RA). Serum from healthy and RA patients met the criteria of ± 20% recovery from nominal at all levels tested. Given the assay LOD of 0.05-0.1 pg/mL and the acceptable recovery data, the LLOQ in matrix was set at 0.1 pg/mL. Accuracy in CSF was also within ± 20% of the nominal spike