A.L. McNulty, B.T. Estes, R.E. Wilusz, J.B. Weinberg, F. Guilak

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Presentation transcript:

Dynamic loading enhances integrative meniscal repair in the presence of interleukin-1 A.L. McNulty, B.T. Estes, R.E. Wilusz, J.B. Weinberg, F. Guilak Osteoarthritis and Cartilage Volume 18, Issue 6, Pages 830-838 (June 2010) DOI: 10.1016/j.joca.2010.02.009 Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 MMP activity in the media of meniscal repair model explants that were exposed to no strain, strain, no strain+IL-1, or strain+IL-1. The strains tested were 1% (top panel), 10% (middle panel), and 26% (bottom panel). The MMP activity is expressed as fluorescence units (FU)/mg wet weight+standard error. *: P<0.05 compared to all other treatments. Osteoarthritis and Cartilage 2010 18, 830-838DOI: (10.1016/j.joca.2010.02.009) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 ADAMTS-4 activity in the media of meniscal repair model explants that were exposed to no strain, strain, no strain+IL-1, or strain+IL-1. The strains tested were 1% (top panel), 10% (middle panel), and 26% (bottom panel). The aggrecanase activity is expressed as fluorescence units (FU)/mg wet weight+standard error. Osteoarthritis and Cartilage 2010 18, 830-838DOI: (10.1016/j.joca.2010.02.009) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 S-GAG release into the media of meniscal repair model explants that were exposed to no strain, strain, no strain+IL-1, or strain+IL-1. The strains tested were 1% (top panel), 10% (middle panel), and 26% (bottom panel). The S-GAG release is expressed as μg S-GAG/mg wet weight+standard error. *: P≤0.005 compared to all other treatments. +: P<0.01 compared to no strain and strain. Osteoarthritis and Cartilage 2010 18, 830-838DOI: (10.1016/j.joca.2010.02.009) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 NO release into the media of meniscal repair model explants that were exposed to no strain, strain, no strain+IL-1, or strain+IL-1. The strains tested were 1% (top panel), 10% (middle panel), and 26% (bottom panel). The NO release is expressed as μmol/g wet weight+standard error. *: P<0.005 compared to all other treatments. +: P<0.05 compared to no strain. Osteoarthritis and Cartilage 2010 18, 830-838DOI: (10.1016/j.joca.2010.02.009) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Integrative shear strength of repair for meniscal repair model explants that were exposed to no strain, strain, no strain+IL-1, or strain+IL-1. The strains tested were 1% (top panel), 10% (middle panel), and 26% (bottom panel). The shear strength of repair is expressed as kPa+standard error. *: P<0.05 compared to all other treatments. #: P<0.005 compared to no strain and no strain+IL-1. +: P<0.01 compared to 26% strain+IL-1. Osteoarthritis and Cartilage 2010 18, 830-838DOI: (10.1016/j.joca.2010.02.009) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Histological images of paraffin embedded meniscal repair model explants stained with Hematoxylin to identify the cell nuclei (black), fast green (green) to identify the collagen fibers, and Safranin O (red) to stain proteoglycans. Samples were incubated with no IL-1 or 100pg/mL IL-1 and subjected to 0%, 1%, 10%, or 26% strain. Scale bar is equal to 100μm. Osteoarthritis and Cartilage 2010 18, 830-838DOI: (10.1016/j.joca.2010.02.009) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions