Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded.

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Presentation transcript:

Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study Richard Scott, M.D., Emre Seli, M.D., Kathy Miller, B.S., Denny Sakkas, Ph.D., Katherine Scott, B.S., David H. Burns, Ph.D. Fertility and Sterility Volume 90, Issue 1, Pages 77-83 (July 2008) DOI: 10.1016/j.fertnstert.2007.11.058 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Raman spectra of spent embryo culture media. (A) Plot of 44 individual spectra from each sample used in this study. (B) Mean centered spectra demonstrating the spectral differences between the spent media of those that implanted compared with those that failed to implant. Fertility and Sterility 2008 90, 77-83DOI: (10.1016/j.fertnstert.2007.11.058) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Viability indexes calculated from Raman biospectroscopic analysis of blinded spent media samples that had contained embryos with known implantation potential (A). Viability indexes are significantly different from media collected on cycle day 3 compared with media collected on cycle day 5 (P<.0001) (B). Distribution of viability indexes on spent media collected on cycle day 3 demonstrating a significant difference in those embryos with 0 and 100% implantation rates (P<.03). Each point represents an individual sample, whereas different colors represent different patients (C). Distribution of viability indexes on spent media collected on cycle day 5 (blastocyst) demonstrating significant differences between those embryos with 0 and 100% implantation rates (P<.009). Each point represents an individual sample, whereas different colors represent different patients. Fertility and Sterility 2008 90, 77-83DOI: (10.1016/j.fertnstert.2007.11.058) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Receiver operator characteristic (ROC) curves of viability indexes and clinical outcome. Overall accuracy for predicting outcome using the thresholds derived from the ROC analysis was 80.4% (A). ROC curve generated from 33 day 3 spent media samples. Overall diagnostic accuracy for predicting implantation and delivery was 76% (B). ROC curve generated from 8 day 5 spent media samples. Overall diagnostic accuracy was 100%. Fertility and Sterility 2008 90, 77-83DOI: (10.1016/j.fertnstert.2007.11.058) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions