Hermine Schlagbauer-Wadl, Marieke Griffioen, Andrea van Elsas, Peter I

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Influence of Increased c-Myc Expression on the Growth Characteristics of Human Melanoma Hermine Schlagbauer-Wadl, Marieke Griffioen, Andrea van Elsas, Peter I. Schrier, Tom Pustelnik, Hans-Georg Eichler, Klaus Wolff, Hubert Pehamberger, Burkhard Jansen Journal of Investigative Dermatology Volume 112, Issue 3, Pages 332-336 (March 1999) DOI: 10.1046/j.1523-1747.1999.00506.x Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Western blot analysis of c-myc transfectants. Expression of c-Myc (B) and HSP70 (A) in cultured human melanoma cells determined by western blotting. Ten, 20, and 30 μg of total protein of myc (7.1) were loaded in lanes 1–3, respectively. In lanes 4 and 5, 30 μg of myc (7.4) and IGR39D-neo protein were loaded. Journal of Investigative Dermatology 1999 112, 332-336DOI: (10.1046/j.1523-1747.1999.00506.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Growth rates of c-myc transfectants and vector control.In vitro growth of c-myc transfected human melanoma cells compared with vector control. Cell numbers were determined by Coulter Counter and each time point represents quadruplicate wells. IGR39D-neo (○), myc (7.4) (▿), and myc (7.1) (▵). SD shown as error bars. Journal of Investigative Dermatology 1999 112, 332-336DOI: (10.1046/j.1523-1747.1999.00506.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Colony formation in soft agar. Increased c-Myc expression alters anchorage-independent growth of human melanoma cells in vitro. The number of colonies in soft agar larger than 0.1 mm in diameter was evaluated by light microscopy 4 wk after plating. Results are from quadruplicate experiments. SD shown as error bars. Journal of Investigative Dermatology 1999 112, 332-336DOI: (10.1046/j.1523-1747.1999.00506.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Directed cell movement mediated by chemotactic factors. (A) Chemotaxis assay with 3T3 fibroblast conditioned medium used as chemoattractant. Number of cells attached to the lower surface of the filter per high power field (×400) are shown ±SD, p < 0.05. (B) Chemotaxis assay with melanoma cell conditioned medium. Number of cells attached to the lower surface of the filter per high power field (×400) are shown ±SD, p < 0.05. Journal of Investigative Dermatology 1999 112, 332-336DOI: (10.1046/j.1523-1747.1999.00506.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Tumor growth in SCID mice. Mean primary tumor weight of human melanomas grown in SCID mice (p < 0.005). SD shown as error bars. Journal of Investigative Dermatology 1999 112, 332-336DOI: (10.1046/j.1523-1747.1999.00506.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Tumor-induced cachexia. Mean body weight of SCID mice (p < 0.005) injected with human melanoma cells. SD shown as error bars. Journal of Investigative Dermatology 1999 112, 332-336DOI: (10.1046/j.1523-1747.1999.00506.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 Morphology of human melanomas grown in SCID mice. Photomicrographs of sections of human melanoma (A), a para-aortal lymph node (B), and the lung (C) of a representative SCID mouse injected with myc (7.1) melanoma cells. Hematoxylin and eosin stain, scale bar: (A, B) 12 μm, (C) 300 μm. Journal of Investigative Dermatology 1999 112, 332-336DOI: (10.1046/j.1523-1747.1999.00506.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions