Significance of the serum CTX-II level in an osteoarthritis animal model: a 5-month longitudinal study M.E. Duclos, O. Roualdes, R. Cararo, J.C. Rousseau,

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Significance of the serum CTX-II level in an osteoarthritis animal model: a 5-month longitudinal study M.E. Duclos, O. Roualdes, R. Cararo, J.C. Rousseau, T. Roger, D.J. Hartmann Osteoarthritis and Cartilage Volume 18, Issue 11, Pages 1467-1476 (November 2010) DOI: 10.1016/j.joca.2010.07.007 Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Macroscopic articular cartilage, stained with India ink, representative of different kinds of macroscopic lesions. A: Normal cartilage, no uptake of India ink; B: fibrillation in lateral femoral condyle (minimal uptake of India ink, with surface irregularities) and fissuration (evident dark patches of ink uptake) in medial femoral condyle; C: fissuration in lateral femoral condyle and ulceration (area demonstrating visible bone) in medial femoral condyle; D: ulceration in both femoral condyles. Osteoarthritis and Cartilage 2010 18, 1467-1476DOI: (10.1016/j.joca.2010.07.007) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Representative histological sections stained with Safranin O-light green and illustrating cartilage lesions. A: Normal articular cartilage with intact surface of a lateral tibia, Magnification 12,5×; B: Loss of cartilage matrix49 and fibrillation in a medial femur, Magnification 50×; C: Clusters of cells in a fibrillation with loss of Safranine O-light green staining lesion in a medial femur, Magnification 200×; D: Erosion of cartilage matrix to subchondral bone in a medial femur, Magnification 25×; E: Junction between both ulceration and cartilage zones medial femur, Magnification 50×. Osteoarthritis and Cartilage 2010 18, 1467-1476DOI: (10.1016/j.joca.2010.07.007) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Standard curves in the normal and modified protocols for the measurement of CTX-II. Osteoarthritis and Cartilage 2010 18, 1467-1476DOI: (10.1016/j.joca.2010.07.007) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Histomorphological score of ACLT and unoperated adult rabbits for the medial (A), lateral (B) and total (C) knee compartments. Box plots show the median, first and third quartiles and the smallest and largest observations. N=5 for the operated group and N=4 for the unoperated group. Osteoarthritis and Cartilage 2010 18, 1467-1476DOI: (10.1016/j.joca.2010.07.007) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Cartilage thickness of ACLT and unoperated adult rabbits for the medial (A), lateral (B) and total (C) knee compartments. Box plots show the median, first and third quartiles and the smallest and largest observations. N=5 for the operated group and N=4 for the unoperated group. Osteoarthritis and Cartilage 2010 18, 1467-1476DOI: (10.1016/j.joca.2010.07.007) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Evolution of serum CTX-II levels in adult (A: N=5 for the operated group and N=4 for the unoperated group) and young rabbits (B N=9 for the operated group and N=4 for the unoperated group). Data are shown as mean and bars represent 95% confidence intervals. Osteoarthritis and Cartilage 2010 18, 1467-1476DOI: (10.1016/j.joca.2010.07.007) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 Correlations between CTX-II levels at the twelfth (upper graphs) and twentieth (bottom graphs) weeks and histomorphological score (A or C) cartilage thickness (B or D) in the medial compartment of the knee. N=9. Osteoarthritis and Cartilage 2010 18, 1467-1476DOI: (10.1016/j.joca.2010.07.007) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions