High-resolution melting analysis of the spa locus reveals significant diversity within sequence type 93 methicillin-resistant Staphylococcus aureus from.

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High-resolution melting analysis of the spa locus reveals significant diversity within sequence type 93 methicillin-resistant Staphylococcus aureus from northern Australia S.Y.C. Tong, R.A. Lilliebridge, D.C. Holt, M.I. McDonald, B.J. Currie, P.M. Giffard Clinical Microbiology and Infection Volume 15, Issue 12, Pages 1126-1131 (December 2009) DOI: 10.1111/j.1469-0691.2009.02732.x Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 High-resolution melting curves of the glpF 165, aroE 252 and spa loci (c–f). (a) Representative curves corresponding to each allele at glpF 165: C (black) is specific for ST93, and A (green) is specific for CC75. (b) Representative curves for the aroE 252 region: the black curve is specific for sequence type (ST 93. (c) ST93 methicillin-resistant Staphylococcus aureus (ST93-MRSA) showing four curves: curve 1 (black), curve 3 (red), curve 4 (green), and curve 5 (yellow). (d) ST93 methicillin-susceptible S. aureus (ST93-MSSA) showing four curves: curve 1 (black), curve 2 (green), curve 6 (red), and curve 10 (yellow). (e) ST30-MRSA showing three curves: curve 7 (blue), curve 8 (red), and curve 9 (green). (f) ST93-MRSA (black), ST93-MSSA (green) and ST30-MRSA (blue) combined. Clinical Microbiology and Infection 2009 15, 1126-1131DOI: (10.1111/j.1469-0691.2009.02732.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Difference graph of high-resolution melting curves of spa loci. (a) Curves for sequence type (ST) 93 isolates. The mean of the known t202 curves was used as the baseline. The green lines indicate the 95% CIs for the 13 t202 curves, and the thin black lines indicate each t202 curve. The blue lines (AS t202) refer to t202 curves derived from the raw data of the study by Stephens et al. [7], and sit within the 95% CI, whereas part of the t4178 (red) curve sits outside the 95% CI. (b) Curves for ST30 isolates. The mean of the known t019 curves was used as the baseline. The green lines indicate the 95% CIs for the seven t019 curves, and the thin black lines indicate each t019 curve. Clinical Microbiology and Infection 2009 15, 1126-1131DOI: (10.1111/j.1469-0691.2009.02732.x) Copyright © 2009 European Society of Clinical Infectious Diseases Terms and Conditions