G. Kouppari, M. Biendo, G. Laurans, D. Thomas, O. Dechepy, F

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Regional dissemination of Salmonella enterica serovar Enteritidis is season dependent G. Kouppari, M. Biendo, G. Laurans, D. Thomas, O. Dechepy, F. Hamdad-Daoudi, B. Canarelli, F. Eb Clinical Microbiology and Infection Volume 9, Issue 5, Pages 360-369 (May 2003) DOI: 10.1046/j.1469-0691.2003.00529.x Copyright © 2003 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 PFGE pattern after complete digestion of the S. enterica serovar Enteritidis Strains using SpeI restriction enzyme. S. enteritidis isolates numbered 1 to 44. Lane M contains the molecular size marker bacteriophage Lambda ladder. The separation size range is from 50 Kbp to 500 Kbp. Isolates 1-5, 7-9,11, 13-19, 24-30, 34, 36-44 appear to be the same strain (strain A) with isolates 12, 32, 33 and 35 being related strains (strain A1 for isolates 11 and 33; strain A2 for isolate 32 and A3 for isolate 35). Isolates 6, 10, 20-23 and 31 do not appear related to any of others isolates and represent 6 unrelated strains (strains B, C, D, E, F and G respectively). Clinical Microbiology and Infection 2003 9, 360-369DOI: (10.1046/j.1469-0691.2003.00529.x) Copyright © 2003 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 RAPD profiles obtained from amplification of Salmonella enterica serover Enteritidis genomic DNA using AQ12 as primer synthetized by DNA Technologies Eurogentec – Belgium. S. Enteritidis isolates numbered 1 to 44 were differentiated into three distinct RAPD types named H, I and J. RAPD type H included 42 isolates of which 23 (1-5, 7-21 and 23-25) were genetically indistinguishable. M, size marker VI: number of basepairs, 154 to 276 (Boehringer Mannheim, France). Clinical Microbiology and Infection 2003 9, 360-369DOI: (10.1046/j.1469-0691.2003.00529.x) Copyright © 2003 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 RAPD profiles obtained from amplification of Salmonella enterica serovar Enteritidis genomic DNA using Q11 as primer synthetized by DNA Technologies Eurogentec – Belgium. S. Enteritidis isolates numbered 1 to 44 were differentiated into six different patterns: K, L, M, N, O and P. RAPD type K and O consisted of 19 and 21 isolates respectively among which 8 isolates of K (1-4, 7, 8, 10 and 11) were genitically identical and 11 isolates of O (23-26, 28-33 and 44) also were identical. M, size marker VI: number of basepairs, 154 to 276 (Boehringer Mannheim, France). Clinical Microbiology and Infection 2003 9, 360-369DOI: (10.1046/j.1469-0691.2003.00529.x) Copyright © 2003 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 4 The monthly repartition of the isolates with their genomic groups (1-11). From January to June, the predominant GG1 plus the GG2,3 and 4 are observed. From July to December we noted a heterogeneity of genomic groups with GG9 as predominant. Clinical Microbiology and Infection 2003 9, 360-369DOI: (10.1046/j.1469-0691.2003.00529.x) Copyright © 2003 European Society of Clinical Infectious Diseases Terms and Conditions