Outgrowth of passaged strains.

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Outgrowth of passaged strains. Outgrowth of passaged strains. After saving, populations from each passage were grown in fresh LB medium into LTSP. Each panel represents the growth for 5 days of population A (blue diamonds), population B (red triangles), and population C (green circles) compared to growth of the parent strain (black squares). Each panel is labeled with the passage from which the aged populations were grown. Lines represent the average from two technical replicates, and error bars represent the standard deviation between the two replicates. Karin E. Kram et al. mSystems 2017; doi:10.1128/mSystems.00192-16