Signaling of Mechanical Stretch in Human Keratinocytes via MAP Kinases

Slides:

Advertisements

Similar presentations

Nan-Hyung Kim, Ai-Young Lee Journal of Investigative Dermatology

Advertisements

Glutamine Suppresses DNFB-Induced Contact Dermatitis by Deactivating p38 Mitogen– Activated Protein Kinase via Induction of MAPK Phosphatase-1 Otgonzaya.

Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation Stefan W. Stoll, Philip E. Stuart, Sylviane Lambert, Alberto.

Cdc42 Inhibits ERK-Mediated Collagenase-1 (MMP-1) Expression in Collagen-Activated Human Keratinocytes Maryam G. Rohani, Brian K. Pilcher, Peter Chen,

Yeonsue Jang, Sang H. Jeong, Yoon-Hee Park, Hyun C

Keloid Fibroblasts Resist Ceramide-Induced Apoptosis by Overexpression of Insulin- Like Growth Factor I Receptor Hiroshi Ishihara, Hiroshi Yoshimoto,

Activators of Peroxisome Proliferator-Activated Receptors Protect Human Skin from Ultraviolet-B-Light-Induced Inflammation Stefan Kippenberger, Marcella.

Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes Genji Imokawa, Yutaka Takagi,

Human Keratinocytes Express Functional CD14 and Toll-Like Receptor 4

Plakoglobin Deficiency Protects Keratinocytes from Apoptosis

H2O2 Accumulation by Catalase Reduction Changes MAP Kinase Signaling in Aged Human Skin In Vivo Mi Hee Shin, Gi-eun Rhie, Yeon Kyung Kim, Chi-Hyun Park,

Daisuke Suzuki, Makoto Senoo Journal of Investigative Dermatology

EGFR Regulates the Expression of Keratinocyte-Derived Granulocyte/Macrophage Colony-Stimulating Factor In Vitro and In Vivo Francesca Mascia, Christophe.

Toshiyuki Ozawa, Daisuke Tsuruta, Jonathan C. R

Low Concentrations of Curcumin Induce Growth Arrest and Apoptosis in Skin Keratinocytes Only in Combination with UVA or Visible Light Jadranka Dujic,

Histamine Contributes to Tissue Remodeling via Periostin Expression

Stefan W. Stoll, Jessica L. Johnson, Yong Li, Laure Rittié, James T

IGF-II-Mediated COX-2 Gene Expression in Human Keratinocytes Through Extracellular Signal-Regulated Kinase Pathway Hye Jung Kim, Tae-Yoon Kim Journal.

Epithelial Cells Promote Fibroblast Activation via IL-1α in Systemic Sclerosis Nima Aden, Anna Nuttall, Xu Shiwen, Patricia de Winter, Andrew Leask, Carol.

Γ-Aminobutyric Acid (A) Receptor Agonists Accelerate Cutaneous Barrier Recovery and Prevent Epidermal Hyperplasia Induced by Barrier Disruption Mitsuhiro.

Bernd Rebholz, Kai Kehe, Thomas Ruzicka, Rudolf A. Rupec

The Proteinase-Activated Receptor-2 Mediates Phagocytosis in a Rho-Dependent Manner in Human Keratinocytes Glynis Scott, Sonya Leopardi, Lorelle Parker,

Anti-Inflammatory Activity of Sertaconazole Nitrate Is Mediated via Activation of a p38– COX-2–PGE2 Pathway Runa Sur, Jeffrey M. Babad, Michelle Garay,

Induction of Adipose Differentiation Related Protein and Neutral Lipid Droplet Accumulation in Keratinocytes by Skin Irritants Emmanuela Corsini, PhD,

S100A15, an Antimicrobial Protein of the Skin: Regulation by E

Volume 56, Issue 5, Pages (November 1999)

David Russell, Heike Ross, E Birgitte Lane

Stratifin-Induced Matrix Metalloproteinase-1 in Fibroblast Is Mediated by c-fos and p38 Mitogen-Activated Protein Kinase Activation Eugene Lam, Runhangiz.

All-Trans-Retinoic Acid Induces Interleukin-8 via the Nuclear Factor-κB and p38 Mitogen-Activated Protein Kinase Pathways in Normal Human Keratinocytes

Reciprocal Regulation of Thymus and Activation-Regulated Chemokine/Macrophage- Derived Chemokine Production by Interleukin (IL)-4/IL-13 and Interferon-γ.

Rosiglitazone Inhibits Proliferation, Motility, and Matrix Metalloproteinase Production in Keratinocytes Narasimharao Bhagavathula, Kamalakar C. Nerusu,

Kellie J. White, Vincent J. Maffei, Marvin Newton-West, Robert A

Akihiro Tada Journal of Investigative Dermatology

Activation and Translocation of p38 Mitogen-Activated Protein Kinase After Stimulation of Monocytes With Contact Sensitizers Pia Brand, Sibylle Plochmann,

Keratins Stabilize Hemidesmosomes through Regulation of β4-Integrin Turnover Kristin Seltmann, Fang Cheng, Gerhard Wiche, John E. Eriksson, Thomas M.

Human Keratinocytes Respond to Osmotic Stress by p38 Map Kinase Regulated Induction of HSP70 and HSP27 M. Garmyn, A. Pupe Journal of Investigative Dermatology

Arsenic Induces Tumor Necrosis Factor α Release and Tumor Necrosis Factor Receptor 1 Signaling in T Helper Cell Apoptosis Hsin-Su Yu, Gwo-Shing Chen

Transforming Growth Factor β1 Induces Apoptosis in Normal Melanocytes but not in Nevus Cells Grown in Type I Collagen Gel Tuomo Alanko Journal of Investigative.

Retinoid-Induced Epidermal Hyperplasia Is Mediated by Epidermal Growth Factor Receptor Activation Via Specific Induction of its Ligands Heparin-Binding.

Heat Shock-Induced Matrix Metalloproteinase (MMP)-1 and MMP-3 Are Mediated through ERK and JNK Activation and via an Autocrine Interleukin-6 Loop Chi-Hyun.

Hydroxychloroquine Modulates Metabolic Activity and Proliferation and Induces Autophagic Cell Death of Human Dermal Fibroblasts Bettina Ramser, Agatha.

Mitogen- and Stress-Activated Protein Kinase 2 and Cyclic AMP Response Element Binding Protein are Activated in Lesional Psoriatic Epidermis Anne T.

James Gailit, Mary J. Marchese, Richard R. Kew, Barry L. Gruber

High Calcium, ATP, and Poly(I:C) Augment the Immune Response to β-Glucan in Normal Human Epidermal Keratinocytes Carren Sy Hau, Yayoi Tada, Sayaka Shibata,

Thrombospondin-1 Plays a Critical Role in the Induction of Hair Follicle Involution and Vascular Regression During the Catagen Phase Kiichiro Yano, Michael.

Interferon-γ, a Strong Suppressor of Cell Proliferation, Induces Upregulation of Keratin K6, One of the Inflammatory- and Proliferation-Associated Keratins

Mechanical Stretching In Vitro Regulates Signal Transduction Pathways and Cellular Proliferation in Human Epidermal Keratinocytes Shoichiro Yano, Mayumi.

IL-17A Upregulates Keratin 17 Expression in Keratinocytes through STAT1- and STAT3- Dependent Mechanisms Xiaowei Shi, Liang Jin, Erle Dang, Ting Chang,

Insulin-Like Growth Factor-II Regulates the Expression of Vascular Endothelial Growth Factor by the Human Keratinocyte Cell Line HaCaT Yoo-Wook Kwon,

Post-Transcriptional Regulation of UV Induced TNF-α Expression

1α,25-Dihydroxyvitamin D3 Stimulates Activator Protein 1 DNA-Binding Activity by a Phosphatidylinositol 3-Kinase/Ras/MEK/Extracellular Signal Regulated.

Induction of RANTES by TWEAK/Fn14 Interaction in Human Keratinocytes

Helium–Neon Laser Irradiation Stimulates Migration and Proliferation in Melanocytes and Induces Repigmentation in Segmental-Type Vitiligo Hsin-Su Yu,

Keratinocytes Inhibit Expression of Connective Tissue Growth Factor in Fibroblasts In Vitro by an Interleukin-1α-Dependent Mechanism Daniel Nowinski,

Ligation of the β4 Integrin Triggers Adhesion Behavior of Human Keratinocytes by an “Inside-out” Mechanism Stefan Kippenberger, Stefan Loitsch, Jutta.

Chemical Chaperones Protect Epidermolysis Bullosa Simplex Keratinocytes from Heat Stress–Induced Keratin Aggregation: Involvement of Heat Shock Proteins.

Regulation of Tissue Factor in Microvascular Dermal Endothelial Cells

Interleukin 6 Indirectly Induces Keratinocyte Migration

Human Leukocyte Elastase Induces Keratinocyte Proliferation by Epidermal Growth Factor Receptor Activation Ulf Meyer-Hoffert, Jana Wingertszahn, Oliver.

Loss of Keratin 10 Leads to Mitogen-activated Protein Kinase (MAPK) Activation, Increased Keratinocyte Turnover, and Decreased Tumor Formation in Mice

CD40 Ligation Alters the Cell Cycle of Differentiating Keratinocytes

Thiol Antioxidants Block the Activation of Antigen-Presenting Cells by Contact Sensitizers Stefanie Bruchhausen, Sabine Zahn, Elke Valk, Jürgen Knop,

Plexin C1, A Receptor for Semaphorin 7A, Inactivates Cofilin and Is a Potential Tumor Suppressor for Melanoma Progression Glynis A. Scott, Lindy A. McClelland,

Coupling of Contact Sensitizers to Thiol Groups is a Key Event for the Activation of Monocytes and Monocyte-Derived Dendritic Cells Detlef Becker, Elke.

Hyun Jeong Park, Hee Jung Kim, Jun Young Lee, Baik Kee Cho, Richard L

Runa Sur, Peter A. Lyte, Michael D. Southall

Roland Houben, Sonja Ortmann, David Schrama, Marco J

The Activity of Caspase-1 Is Increased in Lesional Psoriatic Epidermis

Mechanism of Thymus- and Activation-Regulated Chemokine (TARC)/CCL17 Production and its Modulation by Roxithromycin Mayumi Komine, Takashi Kakinuma,

Fibroblast Growth Factor 10 Induces Proliferation and Differentiation of Human Primary Cultured Keratinocytes Cinzia Marchese, Alessandra Felici, Vincenzo.

Presentation transcript:

Signaling of Mechanical Stretch in Human Keratinocytes via MAP Kinases Stefan Kippenberger, August Bernd, Maike Guschel, Jutta Müller, Roland Kaufmann Journal of Investigative Dermatology Volume 114, Issue 3, Pages 408-412 (March 2000) DOI: 10.1046/j.1523-1747.2000.00915.x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 The stretching device. Cells are grown in the rectangular box on top of the silicone sheets. (A) An overall view of the stretching device. The silicone chambers can be extended by using the right-hand lever. (B) On the left the chamber shows the relaxed state; on the right the chamber is stretched to 10% of the initial length. Journal of Investigative Dermatology 2000 114, 408-412DOI: (10.1046/j.1523-1747.2000.00915.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Mechanical stretch induces s-phases. Stretching of HaCaT cells for 24 h caused an upregulation of BrdU-positive cells to 239% as detected by a commercial BrdU ELISA. Each bar represents the mean of 12 independent experiments given as a percentage ±SD. The results show a high statistical significance (p <0.0001). Journal of Investigative Dermatology 2000 114, 408-412DOI: (10.1046/j.1523-1747.2000.00915.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Mechanical stretch activates MAP kinase ERK1/2 and SAPK/JNK. Time course of MAP kinase activation after a single 5 min stretch in HaCaT cell and normal human keratinocytes. (A) Activity of ERK1/2, represented by in vitro phosphorylation of Elk 1, peaks immediately after stimulation. The basal activity is restored already after 30 min. (B) p38 activation observed by a phospho specific p38 antibody (Thr180/182) shows no activity changes. (C) SAPK/JNK activity determined by in vitro phosphorylation of c-JUN during a time of 180 min. Maximum induction is at 60 min, with a moderate decline of activity. The blot shows representative results (n = 3). Journal of Investigative Dermatology 2000 114, 408-412DOI: (10.1046/j.1523-1747.2000.00915.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Effect of cytoskeleton-modifying drugs on ERK1/2 and SAPK/JNK activation in HaCaT cells. (A) Cells were incubated for 2 h with 0.05, 0.1, and 0.2 μg cytochalasin D per ml and tested for ERK1/2 activity and (B) SAPK/JNK activity. (C) Cells were incubated for 5 h with 5, 10, and 20 μg nocodazole per ml and tested for ERK1/2 activity and (D) SAPK/JNK activity. (E) Cells were incubated for 5 h with 25, 50, and 100 mM acrylamide and tested for ERK1/2 activity and (F) SAPK/JNK activity. The blot shows representative results (n = 3). Journal of Investigative Dermatology 2000 114, 408-412DOI: (10.1046/j.1523-1747.2000.00915.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Stretch-induced effects on ERK1/2 and SAPK/JNK activity in HaCaT cells after selective disruption of cytoskeletal elements. The controls are derived from stretched cells without any cytoskeleton manipulation at maximum activation in response to stretch. (A) Cytochalasin D (0.1 μg per ml) and acrylamide (50 mM) treatment shows no change of stretch-induced ERK1/2 activation. Nocodazole treatment (10 μg per ml) shows strong ERK1/2 activation (see also Figure 4c). (B) No alterations of SAPK/JNK activation in response to stretch are observed on treatment with cytochalasin D and acrylamide. Nocodazole causes SAPK/JNK activity upregulation (see also Figure 4d) (n = 3). Journal of Investigative Dermatology 2000 114, 408-412DOI: (10.1046/j.1523-1747.2000.00915.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Effects of blocking β1 antibodies on cell morphology of HaCaT cells grown on silicone dishes. Cells were stained with methylene blue in order to enhance the contrast to the opaque cell support. The left half shows cells in the control state. On the right half the effect of incubation with β1-integrin antibodies is shown. Colonies become dissociated and cell-cell contacts are diminished. Journal of Investigative Dermatology 2000 114, 408-412DOI: (10.1046/j.1523-1747.2000.00915.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 Blocking of β1-integrins attenuates stretch-induced ERK1/2 activation. HaCaT cells were treated with functional blocking antibody directed towards β1-integrin subunits for 24 h. The characteristic activation of ERK1/2 induced by a single 5 min stretch was attenuated in cultures treated with β1-integrin antibodies (n = 3). Journal of Investigative Dermatology 2000 114, 408-412DOI: (10.1046/j.1523-1747.2000.00915.x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions