Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis Peter Valentin Tomazic,

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Presentation transcript:

Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis Peter Valentin Tomazic, MD, Ruth Birner-Gruenberger, PhD, Anita Leitner, Britta Obrist, BSc, Stefan Spoerk, Doris Lang-Loidolt, MD Journal of Allergy and Clinical Immunology Volume 133, Issue 3, Pages 741-750 (March 2014) DOI: 10.1016/j.jaci.2013.09.040 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Differences in mean SCs (logarithmic scale) between patients with allergic rhinitis and healthy control subjects for proteins (n = 51) with an SC of 4 or greater in either group. Proteins marked with an asterisk are significantly different (∗P < .05, Mann-Whitney U test). Journal of Allergy and Clinical Immunology 2014 133, 741-750DOI: (10.1016/j.jaci.2013.09.040) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 A, Enrichment analysis of biological processes, obtained by using BINGO software, of significantly enriched biological processes of proteins present in patients with allergic rhinitis (n = 12) compared with the total human proteome. Nodes surrounded by a black rectangle indicate biological processes exclusively found in patients with allergic rhinitis (n = 4) compared with healthy control subjects. The color bar in the right lower quadrant indicates level of significance from low (yellow) to high (orange). Statistical analysis was performed with a hypergeometric test. A P value of less than .05 was considered significant. B, Enrichment analysis of biological processes, obtained by using BINGO software, of significantly enriched biological processes of proteins present in healthy control subjects (n = 14) compared with the total human proteome. Nodes surrounded by a black rectangle indicate biological processes exclusively found in healthy control subjects (n = 6) compared with patients with allergic rhinitis. The color bar in the right lower quadrant indicates level of significance. Statistical analysis was performed with a hypergeometric test. A P value of less than .05 was considered significant. Journal of Allergy and Clinical Immunology 2014 133, 741-750DOI: (10.1016/j.jaci.2013.09.040) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Western blot analysis of selected proteins. Five proteins, APOA2 (A), A2M (B), SERPINA1 (C), C3 (D), and HP (E), were analyzed. Panel I shows the respective immunoblots of 5 healthy control subjects and 5 patients with allergic rhinitis. Estimated molecular weights in comparison with a protein standard were 12 kDa for APOA2, 160 kDa for A2M, 55 kDa for SERPINA1, 120 kDa for C3, and 50 kDa for HP. Panel II shows the densitometric analysis of the immunoblots. Means and SEMs are compared in healthy subjects versus allergic patients for each protein. Journal of Allergy and Clinical Immunology 2014 133, 741-750DOI: (10.1016/j.jaci.2013.09.040) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions