Skeletal myoblast sheet transplantation improves the diastolic function of a pressure- overloaded right heart Takaya Hoashi, MD, Goro Matsumiya, MD, PhD,

Slides:

Advertisements

Similar presentations

Manuel J. Antunes, MD, PhD, DSc

Advertisements

Aortic valve replacement in low-flow, low-gradient aortic stenosis: Left ventricular ejection fraction matters Victor Dayan, MD, PhD, Philippe Pibarot,

Is individualized mitral valve repair in the future?

Therapeutic Effect of Midkine on Cardiac Remodeling in Infarcted Rat Hearts Shinya Fukui, MD, Satoru Kitagawa-Sakakida, MD, PhD, Sin Kawamata, MD, PhD,

Sirtuin1 Regulates the Stem Cell Therapeutic Effects on Regenerative Capability for Treating Severe Heart Failure in a Juvenile Animal Model Hideto Ozawa,

An unusual case of severe aortic valve stenosis in an adult caused by aortic valve inflammatory myofibroblastic tumor Dong Zhao, MD, Chunsheng Wang,

A slow-releasing form of prostacyclin agonist (ONO1301SR) enhances endogenous secretion of multiple cardiotherapeutic cytokines and improves cardiac function.

Tissue-engineered stent-graft integrates with aortic wall by recruiting host tissue into graft scaffold Mugiho Takeuchi, MD, Toru Kuratani, MD, PhD,

Long-term biventricular support with rotary blood pumps in a patient with a noncontractile heart Taichi Sakaguchi, MD, Shunsuke Saito, MD, Daisuke Yoshioka,

Combined Strategy Using Myoblasts and Hepatocyte Growth Factor in Dilated Cardiomyopathic Hamsters Haruhiko Kondoh, MD, Yoshiki Sawa, MD, Norihide Fukushima,

Reorganization of cytoskeletal proteins and prolonged life expectancy caused by hepatocyte growth factor in a hamster model of late-phase dilated cardiomyopathy

Ultrasound, endoscopy, and the recurrent nerve

Biodegradable vs Nonbiodegradable Cardiac Support Device for Treating Ischemic Cardiomyopathy in a Canine Heart Mutsunori Kitahara, MD, Shigeru Miyagawa,

Intrinsic cardiac stem cells are essential for regeneration

Targeted overexpression of leukemia inhibitory factor to preserve myocardium in a rat model of postinfarction heart failure Mark F. Berry, MD, Timothy.

Centers for Disease Control “increased-risk” organ donor: Not so risky? Francis D. Pagani, MD, PhD The Journal of Thoracic and Cardiovascular Surgery

Vascular endothelial growth factor transgene expression in cell-transplanted hearts Terrence M. Yau, MD, MSc, Guangming Li, MD, Richard D Weisel, MD,

Abracadabra I, II…HeartMate 3?

Alternation of left ventricular load by a continuous-flow left ventricular assist device with a native heart load control system in a chronic heart failure.

Right ventricular failure secondary to chronic overload in congenital heart diseases: Benefits of cell therapy using human embryonic stem cell–derived.

Victor van Berkel, MD, PhD

William M. DeCampli, MD, PhD

Philippe Menasché, MD, PhD

Left ventricular apical papillary fibroelastoma

Increased amount of atrial fibrosis in patients with atrial fibrillation secondary to mitral valve disease Guillaume S.C. Geuzebroek, MD, Shirley C.M.

The lord of the rings Antonio Miceli, MD, PhD

A self-renewing, tissue-engineered vascular graft for arterial reconstruction Kei Torikai, MD, Hajime Ichikawa, MD, PhD, Koichiro Hirakawa, MS, Goro Matsumiya,

Is individualized mitral valve repair in the future?

Novel regenerative therapy using cell-sheet covered with omentum flap delivers a huge number of cells in a porcine myocardial infarction model Yasuhiro.

Jared P. Beller, MD, Irving L. Kron, MD

Acute left ventricular failure after bilateral lung transplantation for idiopathic pulmonary arterial hypertension Tom Verbelen, MD, Sophie Van Cromphaut,

Bilateral sympathectomy improves postinfarction left ventricular remodeling and function Fernando Luiz Zanoni, DVM, PhD, Rafael Simas, PhD, Raphael Grillo.

In situ tissue regeneration using a novel tissue-engineered, small-caliber vascular graft without cell seeding Takenori Yokota, MD, Hajime Ichikawa,

Aditya K. Kaza, MD The Journal of Thoracic and Cardiovascular Surgery

Anish Krishnan, BSc, Douglas Drak, BSc, Shisan Bao, PhD, David S

The assessment of cost effectiveness and the effectiveness of cost assessment in cardiothoracic surgery Vinay Badhwar, MD The Journal of Thoracic and.

It's not “just a shunt” but sometimes it should be…

Experience from an American Association for Thoracic Surgery Foundation for Thoracic Surgery Training Fellowship: Lung transplantation in Toronto General.

Takeyoshi Ota, MD, PhD, David Schwartzman, MD, Marco A. Zenati, MD

A first start for lung transplantation?

Right ventricular failure after cardiac surgery: Why can't right ventricular assist device support fix the problem? Francis D. Pagani, MD, PhD The Journal.

Role of myocardial hypertrophy on acute and chronic right ventricular performance in relation to chronic volume overload in a porcine model: Relevance.

Get it right the first time

A fusion protein of hepatocyte growth factor enhances reconstruction of myocardium in a cardiac patch derived from porcine urinary bladder matrix Takeyoshi.

Layered implantation of myoblast sheets attenuates adverse cardiac remodeling of the infarcted heart Naosumi Sekiya, MD, Goro Matsumiya, MD, PhD, Shigeru.

Leora B. Balsam, MD, Abe DeAnda, MD

Fueling the heart: Shifting the myocardial metabolome by targeting endothelial autophagy Nicholas D. Andersen, MD The Journal of Thoracic and Cardiovascular.

Tissue-Engineered Cardiac Constructs for Cardiac Repair

A fate worse than death Jennifer S. Lawton, MD

Grafted skeletal myoblast sheets attenuate myocardial remodeling in pacing-induced canine heart failure model Hiroki Hata, MD, Goro Matsumiya, MD, PhD,

Chronic septal infarction confers right ventricular protection during mechanical left ventricular unloading James Mau, BSc, MB, BS, Stuart Menzie, MB,

The Ross procedure: Time to reevaluate the guidelines

The effect of gene transfer with hepatocyte growth factor for pulmonary vascular hypoplasia in neonatal porcine model Masamichi Ono, MD, PhD, Yoshiki.

Passing the torch The Journal of Thoracic and Cardiovascular Surgery

Physiologic left ventricular reconstruction: Shape, function, and time recaptured Gerald D. Buckberg, MD The Journal of Thoracic and Cardiovascular Surgery

Advancing Cor Triatriatum in Fontan Circulation

The origins of open heart surgery at the University of Minnesota 1951 to 1956 Richard A. DeWall, MD The Journal of Thoracic and Cardiovascular Surgery

Elevation of plasma angiotensin with the development of pulmonary arteriovenous malformations after cavopulmonary shunt Masamichi Ono, MD, PhD, Norihide.

The Journal of Thoracic and Cardiovascular Surgery

Discussion The Journal of Thoracic and Cardiovascular Surgery

The future of cardiac surgery training: A survival guide

The continuing challenge of congenital heart disease in China

Transplantation of cryopreserved muscle cells in dilated cardiomyopathy: Effects on left ventricular geometry and function Nobuhisa Ohno, MD, Paul W.M.

Ralph E. Delius, MD The Journal of Thoracic and Cardiovascular Surgery

Should it stay or should it go?

“The more things change…”: The challenges ahead

Did you like Terminator 3 better than Terminator 2

A good chimney requires a good sweep

How do we follow up our patients

Gene transfection of hepatocyte growth factor attenuates the progression of cardiac remodeling in the hypertrophied heart Keiji Iwata, MD, Yoshiki Sawa,

Presentation transcript:

Skeletal myoblast sheet transplantation improves the diastolic function of a pressure- overloaded right heart Takaya Hoashi, MD, Goro Matsumiya, MD, PhD, Shigeru Miyagawa, MD, PhD, Hajime Ichikawa, MD, PhD, Takayoshi Ueno, MD, PhD, Masamichi Ono, MD, PhD, Atsuhiro Saito, PhD, Tatsuya Shimizu, MD, PhD, Teruo Okano, MD, PhD, Naomasa Kawaguchi, PhD, Nariaki Matsuura, MD, PhD, Yoshiki Sawa, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 138, Issue 2, Pages 460-467 (August 2009) DOI: 10.1016/j.jtcvs.2009.02.018 Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Representative pressure–volume loops of the control (A), sham (B), and myoblast sheet transplantation (MST; C) groups under different loading conditions. The slope of the end-systolic pressure–volume relationship is displayed as a black straight line. The correlation of the end-diastolic pressure–volume relationship is displayed as a green monoexponential curve. RVP, Right ventricular pressure; RV, right ventricle. The Journal of Thoracic and Cardiovascular Surgery 2009 138, 460-467DOI: (10.1016/j.jtcvs.2009.02.018) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Macroscopic photographs of hematoxylin and eosin–stained sections showing right ventricular (RV) wall thickening, cavity enlarging, and the intraventricular septum shifting towered the left side in the sham and myoblast sheet transplantation (MST) groups (A and D). Photomicrographs (40×, scale bar = 200 μm) of hematoxylin and eosin–stained sections showed a fibrous organized thin layer on the epicardium in the myoblast sheet transplantation group (B). Photomicrographs (400×, scale bar = 20 μm) of periodic acid–Schiff–stained sections showed significantly hypertrophied ventricular myocytes in the sham and myoblast sheet transplantation groups (C and E). ∗P < .05 (n = 10). The Journal of Thoracic and Cardiovascular Surgery 2009 138, 460-467DOI: (10.1016/j.jtcvs.2009.02.018) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Representative photomicrographs (100×, scale bar = 100 μm) of Factor VIII–stained epicardial layer (A), mid layer (B), and endocardial layer (C). Neovascularization occurred at the epicardial layer in the myoblast sheet transplantation (MST) group (D). ∗P < .05 (n = 10). The Journal of Thoracic and Cardiovascular Surgery 2009 138, 460-467DOI: (10.1016/j.jtcvs.2009.02.018) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Representative photomicrographs of Masson's trichrome–stained transmural layer (A; 40×, scale bar = 200 μm), epicardial layer (B), mid layer (C), and endocardial layer (D; 200×, scale bar = 50 μm) for evaluation of interstitial (E) and endomyocardial (F) fibrosis. ∗P < .05 (n = 10). The Journal of Thoracic and Cardiovascular Surgery 2009 138, 460-467DOI: (10.1016/j.jtcvs.2009.02.018) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 RT-PCR for evaluation of neurohormonal factor gene expression. The results are expressed after normalization for GAPDH. HGF, Hepatocyte growth factor; VEGF, vascular endothelial growth factor. ∗P < .05 (n = 10). The Journal of Thoracic and Cardiovascular Surgery 2009 138, 460-467DOI: (10.1016/j.jtcvs.2009.02.018) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions