Regional Difference of Inflammatory Acne Lesions According to β-Defensin-2 Expression  Dae Kyoung Choi, Zheng Jun Li, In Kyu Chang, Kyung Cheol Sohn, Young Lee, Young Joon Seo, Young Ho Lee, Ge Shi, Chang Deok Kim, Jeung Hoon Lee, Myung Im  Journal of Investigative Dermatology  Volume 134, Issue 7, Pages 2044-2046 (July 2014) DOI: 10.1038/jid.2014.116 Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Regional differences in inflammatory and noninflammatory lesions in acne patients. (a) Areas of each facial region to be evaluated by lesion counts. The boundaries of these areas were determined based on regional variations in sebum secretion. The blue area indicates the U-zone (both the cheeks) and the red area is the T-zone (the forehead, nose, and the chin). (b, c) Numbers of noninflammatory and inflammatory lesions per square centimeter in each zone in 200 acne patients. Data represent the means±SE. Data were analyzed by Student’s t-test (*P<0.05, **P<0.01). The patient has given written informed consent for publication of the image. For details see Supplementary Materials online. Journal of Investigative Dermatology 2014 134, 2044-2046DOI: (10.1038/jid.2014.116) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Expression and role of hBD-2 on human sebaceous glands and sebocytes. (a) Human sebaceous glands from U- and T-zones were assayed for hBD-2 by immunohistochemistry. Bar=100 μm. (b) Human sebocytes from each area were analyzed for hBD-2. Bar=20 μm. (c) Western blotting of hBD-2 protein and (d) reverse transcriptase–PCR analysis of hBD-2 mRNA in sebocytes from each area. (e) The concentrations of hBD-2 from sebcote extractes were measured by ELISA. (f) Propionibacterium acnes was incubated with cell extract, and the growth was calculated by colony counting. (g) After P. acnes stimulation, cell supernatants were analyzed by ELISA. NS, no significant difference. Data represent the means±SE. Data were analyzed by Student’s t-test (*P<0.05, **P<0.01). For details see Supplementary Materials online. Journal of Investigative Dermatology 2014 134, 2044-2046DOI: (10.1038/jid.2014.116) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions