TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells A, BWestern.

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TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells TGF‐β1 treatment induces the chromatin binding of Smad2/3/4 in 4T1 cells A, BWestern blotting analyzed Smads in 4T1 cells treated with recombinant TGF‐β1 proteins. After treatment, the cytoplasm‐, nucleoplasm‐, and chromatin‐binding fractions of TGFβ‐treated 4T1 cells were isolated, respectively, by using commercial sub‐cellular protein fractionation kit for cultured cells (Thermo Scientific #78840). All experimental data were verified in at least two independent experiments. The intensity of each band was quantified using the ImageJ software. Gapdh or p84 was used as a loading control. Relative expression (RE) of protein levels in each sample to control untreated 4T1 cells is indicated. Source data are available online for this figure. Shih‐Chia Huang et al. EMBO Mol Med. 2017;emmm.201606914 © as stated in the article, figure or figure legend