Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis  Li-Ying Yan, Ph.D., Jie Qiao, M.D., Yuan.

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Application of three-dimensional fluorescence in situ hybridization to human preimplantation genetic diagnosis  Li-Ying Yan, Ph.D., Jie Qiao, M.D., Yuan Chen, Ph.D., Jin Huang, M.S., Ping Liu, Ph.D., Qing-Yuan Sun, Ph.D.  Fertility and Sterility  Volume 92, Issue 4, Pages 1492-1495 (October 2009) DOI: 10.1016/j.fertnstert.2008.08.070 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Application of 3D-FISH to analyze arrested human embryonic blastomeres collected on day 3. (A, B) Interphase of blastomeres at three-cell to four-cell stages, showing the two X chromosomes in green. (C, D) Interphase of embryo at one-cell stage, showing one X chromosome in green. Chromosome X-specific probe was labeled green fluorochrome (green), and DNA was stained with DAPI (blue). Bar = 10 μm. Fertility and Sterility 2009 92, 1492-1495DOI: (10.1016/j.fertnstert.2008.08.070) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

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