Deborah N. Burshtyn, Jiyeon Shin, Christopher Stebbins, Eric O. Long

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Adhesion to target cells is disrupted by the killer cell inhibitory receptor Deborah N. Burshtyn, Jiyeon Shin, Christopher Stebbins, Eric O. Long Current Biology Volume 10, Issue 13, Pages 777-780 (June 2000) DOI: 10.1016/S0960-9822(00)00568-6

Figure 1 Formation of YTS–target cell conjugates. Typical flow cytometric data for binding of YTS NK cells to 221-Cw3 target cells after (a) 0 min and (b) 10 min of incubation at 37°C. Green fluorescence on the x axis detects NK cells; red fluorescence on the y axis detects target cells. Current Biology 2000 10, 777-780DOI: (10.1016/S0960-9822(00)00568-6)

Figure 2 (a) Conjugate formation was measured at 37°C for YTS (circles) and YTS-2DL1 (squares) cells with 221-Cw3 (open symbols) and 221-Cw4 cells (filled symbols) cells. (b) Conjugate formation of YTS-2DL1 with 221-Cw3 and 221-Cw4 was measured after 10 min at 37°C in the presence of a control IgM MOPC104E (gray bars) or anti-KIR2DL1 IgM antibody HP-3E4 (white bars). (c) Conjugates were measured as in (a) using YTS lines expressing the chimeric receptors 2DL1–SHP-1 (circles) or 2DL1–SHP-1(RM) (triangles). Current Biology 2000 10, 777-780DOI: (10.1016/S0960-9822(00)00568-6)

Figure 3 Requirements for adhesion and cytolysis by YTS-2DL1. (a–c) Conjugate formation and (d–f) target cell lysis by YTS-2DL1 were measured with 221-Cw3 (open symbols) or 221-Cw4 cells (filled symbols). (a,d) The assays were carried out in the presence of MOPC21 (control) IgG (circles), anti-LFA-1 (triangles), or anti-CD28 (squares) antibodies. Antibody concentration in (a) was 10 μg/ml. (b,e) Assays were carried out in the presence of MOPC21 IgG (circles) and CTLA-4–Ig (squares). (c,f) YTS-2DL1 cells were pretreated with PP1 at 0 μM (circles), 2 μM (triangles), 4 μM (squares) and 8 μM (diamonds). PP1 was included in the lysis assay. Current Biology 2000 10, 777-780DOI: (10.1016/S0960-9822(00)00568-6)