Discrimination and characterization of Sertoli cell-only syndrome in non-obstructive azoospermia using cell-free seminal DDX4 Qiong Yu, Xiuli Gu, Xuejun.

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Presentation transcript:

Discrimination and characterization of Sertoli cell-only syndrome in non-obstructive azoospermia using cell-free seminal DDX4 Qiong Yu, Xiuli Gu, Xuejun Shang, Honggang Li, Chengliang Xiong Reproductive BioMedicine Online Volume 33, Issue 2, Pages 189-196 (August 2016) DOI: 10.1016/j.rbmo.2016.05.001 Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Comparison of DDX4 mRNA between cell-free seminal mRNA (cfs-mRNA) and testicular samples obtained by microdissection testicular sperm extraction (micro-TESE). Of the 19 NOA cases with DDX4 positive in cfs-mRNA, three patients (No. 6, 7, 19) were DDX4 mRNA-negative in their micro-TESE samples. GAPDH = positive control; M = DNA marker; NC = negative control. Reproductive BioMedicine Online 2016 33, 189-196DOI: (10.1016/j.rbmo.2016.05.001) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Three-dimensional representation of the hormones in Sertoli cell-only (SCO) and non-SCO cases, discriminated with DDX4 cell-free seminal mRNA (cfs-mRNA), in NOA patients without known genetic causes (A, n = 172) and with known genetic causes (B, n = 45). Data are presented for cases for which all hormone concentrations were available, and the number of cases is indicated in brackets. Reproductive BioMedicine Online 2016 33, 189-196DOI: (10.1016/j.rbmo.2016.05.001) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions