Volume 16, Issue 5, Pages 490-498 (May 2009) Dissecting Structural and Functional Diversity of the Lantibiotic Mersacidin  Antony N. Appleyard, Shaila Choi, Daniel M. Read, Ann Lightfoot, Steven Boakes, Anja Hoffmann, Ian Chopra, Gabriele Bierbaum, Brian A.M. Rudd, Michael J. Dawson, Jesus Cortes  Chemistry & Biology  Volume 16, Issue 5, Pages 490-498 (May 2009) DOI: 10.1016/j.chembiol.2009.03.011 Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 1 The Structure of Mersacidin and Its Gene Cluster (A) Primary structure of mersacidin indicating the sequence and thioether bridge pattern. (B) Organization of the mersacidin biosynthetic gene cluster. (C) Engineered system for generating mersacidin mutants. Da, dehydroalanine; Ab, 2-aminobutyrate. Chemistry & Biology 2009 16, 490-498DOI: (10.1016/j.chembiol.2009.03.011) Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 2 Mersacidin Substitution Library Under the linear representation of mersacidin, the circles indicate the variants that were detected by LC-MS. Gray circles indicate variants produced at high yield (>10%, versus mersacidin production), and white circles indicate variants produced at trace levels. Da, dehydroalanine; Db, dehydrobutyrine; Ab, 2-aminobutyrate. Chemistry & Biology 2009 16, 490-498DOI: (10.1016/j.chembiol.2009.03.011) Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 3 Mersacidin Insertion Library Under the linear representation of mersacidin, the arrows indicate the positions where residues were inserted, and the circles indicate the insertion variants that were detected by LC-MS. Gray circles indicate variants produced at high yield (>10%, versus mersacidin production), and white circles indicate variants produced at trace levels. Da, dehydroalanine; Db, dehydrobutyrine; Ab, 2-aminobutyrate. Chemistry & Biology 2009 16, 490-498DOI: (10.1016/j.chembiol.2009.03.011) Copyright © 2009 Elsevier Ltd Terms and Conditions