Arthroscopic airbrush assisted cell implantation for cartilage repair in the knee: a controlled laboratory and human cadaveric study T.S. de Windt, L.A.

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Presentation transcript:

Arthroscopic airbrush assisted cell implantation for cartilage repair in the knee: a controlled laboratory and human cadaveric study T.S. de Windt, L.A. Vonk, J.K. Buskermolen, J. Visser, M. Karperien, R.L.A.W. Bleys, W.J.A. Dhert, D.B.F. Saris Osteoarthritis and Cartilage Volume 23, Issue 1, Pages 143-150 (January 2015) DOI: 10.1016/j.joca.2014.09.016 Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Cell viability (average % and 95% confidence interval) for the airbrush and extrusion application (n = 4). Osteoarthritis and Cartilage 2015 23, 143-150DOI: (10.1016/j.joca.2014.09.016) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Confocal microscopy after the airbrush application of chondron laden fibrin glue (n = 4). A homogenous distribution was found with an equal survival in the lower, central and superficial layer. Green and red staining indicated live and dead cells, respectively. Osteoarthritis and Cartilage 2015 23, 143-150DOI: (10.1016/j.joca.2014.09.016) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Glycosaminoglycan (GAG) per DNA (average and 95% confidence interval) for the 21-day fibrin glue cultures (n = 4). Comparison between airbrush and extrusion. Osteoarthritis and Cartilage 2015 23, 143-150DOI: (10.1016/j.joca.2014.09.016) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 A, Modified macroscopic outline score (average and 95% confidence interval) for the different observers (n = 8 specimens). B, Modified macroscopic coverage score (average and 95% confidence interval) for the different observers (n = 8 specimens). Osteoarthritis and Cartilage 2015 23, 143-150DOI: (10.1016/j.joca.2014.09.016) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Pictures showing a full-thickness cartilage defect on the medial femur condyle in a human cadaveric knee with the best defect coverage and outline results after both airbrushing (A) and needle extrusion (B). The worst results for both methods are shown in C and D. Osteoarthritis and Cartilage 2015 23, 143-150DOI: (10.1016/j.joca.2014.09.016) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions