Allergen content in German cockroach extracts and sensitization profiles to a new expanded set of cockroach allergens determine in vitro extract potency.

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Allergen content in German cockroach extracts and sensitization profiles to a new expanded set of cockroach allergens determine in vitro extract potency for IgE reactivity Jill Glesner, BS, Stephanie Filep, BS, Lisa D. Vailes, MS, Sabina Wünschmann, PhD, Martin D. Chapman, PhD, Giovanni Birrueta, BS, April Frazier, PhD, Kyoung Yong Jeong, PhD, Coby Schal, PhD, Leonard Bacharier, MD, Avraham Beigelman, MD, Paula Busse, MD, Véronique Schulten, PhD, Alessandro Sette, Dr Biol Sci, Anna Pomés, PhD Journal of Allergy and Clinical Immunology Volume 143, Issue 4, Pages 1474-1481.e8 (April 2019) DOI: 10.1016/j.jaci.2018.07.036 Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Allergen levels in cockroach extracts. Concentrations of Bla g 1, Bla g 2, and Bla g 5 in the extracts were measured with immunoassays (extract 13 is a negative control). The inset shows Bla g 5 concentrations in nanograms per milliliter. Journal of Allergy and Clinical Immunology 2019 143, 1474-1481.e8DOI: (10.1016/j.jaci.2018.07.036) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Allergen-specific IgE patterns of sensitization. Patterns of IgE sensitization to 8 purified cockroach allergens in a population of 23 patients with cockroach allergy. The last 2 columns show cockroach-specific IgE antibody levels and skin prick test wheal sizes (skin prick tests were not performed for patients 3, 7, 8, 11, and 15). Subjects are ordered by lowest to highest cockroach-specific IgE levels. Journal of Allergy and Clinical Immunology 2019 143, 1474-1481.e8DOI: (10.1016/j.jaci.2018.07.036) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Component analysis of IgE reactivity to 8 cockroach allergens in a US population of cockroach-allergic subjects. Allergen- and cockroach-specific IgE antibody levels from 23 subjects are shown. Long and short horizontal lines indicate geometric means and 95% CIs, respectively. The cutoff level for IgE quantification (0.35 kUA/L) is indicated by the horizontal dotted line. The number of negative results (<0.35 kUA/L) is provided for each allergen under the corresponding cluster of symbols. Journal of Allergy and Clinical Immunology 2019 143, 1474-1481.e8DOI: (10.1016/j.jaci.2018.07.036) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Correlation between cockroach (CR)–specific IgE levels and the sum of specific IgE levels to each of the 8 cockroach allergens for 23 subjects. Plasma cockroach-specific IgE levels and sum of allergen-specific IgE levels were plotted after log10 transformation for normalization of these variables. Journal of Allergy and Clinical Immunology 2019 143, 1474-1481.e8DOI: (10.1016/j.jaci.2018.07.036) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Proportion of allergen-specific IgE levels versus the sum of 8 allergen-specific IgE levels in subjects with cockroach allergy (n = 23). This figure represents a normalization to percentages of data from Fig 2, including data of less than the 0.35 kUA/L threshold (as 0.35 values). Journal of Allergy and Clinical Immunology 2019 143, 1474-1481.e8DOI: (10.1016/j.jaci.2018.07.036) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Inhibition assays to determine in vitro potencies for IgE reactivity of extracts in 5 patients with cockroach allergy. Results are from 4 representative subjects out of 5 analyzed. Plots show means with SDs of duplicates. Reference curves for each of the 3 plates used in the experiment are 9-1, 9-2, and 9-3. Ab, Antibody. Journal of Allergy and Clinical Immunology 2019 143, 1474-1481.e8DOI: (10.1016/j.jaci.2018.07.036) Copyright © 2018 American Academy of Allergy, Asthma & Immunology Terms and Conditions