Prevalence of Intracellular Galectin-1-Expressing Lymphocytes in Umbilical Cord Blood in Comparison with Adult Peripheral Blood Szonja Kollár, Noémi Sándor, Attila Molvarec, Balázs Stenczer, János Rigó, Tivadar Tulassay, Barna Vásárhelyi, Gergely Toldi Biology of Blood and Marrow Transplantation Volume 18, Issue 10, Pages 1608-1613 (October 2012) DOI: 10.1016/j.bbmt.2012.05.008 Copyright © 2012 American Society for Blood and Marrow Transplantation Terms and Conditions
Figure 1 Boxplots representing the prevalence of intracellular galectin-1(Gal-1)-expressing lymphocytes in adult peripheral blood (APB) and umbilical cord blood (UCB). Horizontal line: median, box: interquartile range, whisker: range. *P < .05 for all comparisons. Biology of Blood and Marrow Transplantation 2012 18, 1608-1613DOI: (10.1016/j.bbmt.2012.05.008) Copyright © 2012 American Society for Blood and Marrow Transplantation Terms and Conditions
Figure 2 (A) The 5 × 105 T cells separated from adult peripheral blood (APB) or umbilical cord blood (UCB) were cultivated for 3 days in the presence or absence of 1 μg/mL ConA polyclonal T cell stimulator. After 3 days of culture, 3H-thymidine was added to the wells and incorporation was measured 18 hours later. Data are given as mean CPM values ± SD of quadruplicate samples of 3 independent donors. *P < .05; **P < .01. (B) T cells from APB or UCB were separated and cultivated for 3 days in the presence of 1 μg/mL ConA and with or without 5 μM recombinant human galectin-1 (Gal-1). After 3 days of culture, 3H-thymidine was added to the wells and incorporation was measured 18 hours later. Proliferation without Gal-1 was regarded as 100%. Data are given as percent proliferation ± SD of ConA + Gal-1 treated samples calculated from 3 independent donors. No statistically significant difference was detected compared with ConA treated samples without Gal-1. Biology of Blood and Marrow Transplantation 2012 18, 1608-1613DOI: (10.1016/j.bbmt.2012.05.008) Copyright © 2012 American Society for Blood and Marrow Transplantation Terms and Conditions