Thaned Kangsamaksin, Rebecca J. Morris

Slides:

Advertisements

Similar presentations

Keratinocyte Stem Cells but Not Melanocyte Stem Cells Are the Primary Target for Radiation-Induced Hair Graying Hitomi Aoki, Akira Hara, Tsutomu Motohashi,

Advertisements

Bone Morphogenetic Protein Signaling Suppresses Wound-Induced Skin Repair by Inhibiting Keratinocyte Proliferation and Migration Christopher J. Lewis,

Psoriatic Inflammation Facilitates the Onset of Arthritis in a Mouse Model Mayuko Yamamoto, Kimiko Nakajima, Mikiro Takaishi, Shun Kitaba, Yasuhiro Magata,

Ephrin-A2 and Ephrin-A5 Are Important for the Functional Development of Cutaneous Innervation in a Mouse Model Dulharie T. Wijeratne, Jennifer Rodger,

Ceramide Synthase 4 Regulates Stem Cell Homeostasis and Hair Follicle Cycling Franziska Peters, Susanne Vorhagen, Susanne Brodesser, Kristin Jakobshagen,

Activated Kras Alters Epidermal Homeostasis of Mouse Skin, Resulting in Redundant Skin and Defective Hair Cycling Anandaroop Mukhopadhyay, Suguna R.

Unprocessed Interleukin-36α Regulates Psoriasis-Like Skin Inflammation in Cooperation With Interleukin-1 Katelynn A. Milora, Hangfei Fu, Ornella Dubaz,

Inactivation of the Vitamin D Receptor Enhances Susceptibility of Murine Skin to UV- Induced Tumorigenesis Tara I. Ellison, Molly K. Smith, Anita C. Gilliam,

Upregulation of Inflammatory Cytokines and Oncogenic Signal Pathways Preceding Tumor Formation in a Murine Model of T-Cell Lymphoma in Skin Xuesong Wu,

Figure 3. Effect of HPD on basal cell proliferation in mouse skin

Ectodysplasin A Pathway Contributes to Human and Murine Skin Repair

Overexpression of Hedgehog Signaling Is Associated with Epidermal Tumor Formation in Vitamin D Receptor–Null Mice Arnaud E. Teichert, Hashem Elalieh,

Human Papillomavirus E6/E7 Oncogenes Promote Mouse Ear Regeneration by Increasing the Rate of Wound Re-epithelization and Epidermal Growth Concepción.

Impaired Keratinocyte Proliferative and Clonogenic Potential in Transgenic Mice Overexpressing σ in the Epidermis Francesca Cianfarani, Silvia.

Protective Effect of Kit Signaling for Melanocyte Stem Cells against Radiation-Induced Genotoxic Stress Hitomi Aoki, Akira Hara, Tsutomu Motohashi, Takahiro.

Overexpression of PRAS40T246A in the Proliferative Compartment Suppresses mTORC1 Signaling, Keratinocyte Migration, and Skin Tumor Development Okkyung.

Hair Cycle Resting Phase Is Regulated by Cyclic Epithelial FGF18 Signaling Miho Kimura-Ueki, Yuko Oda, Junko Oki, Akiko Komi-Kuramochi, Emi Honda, Masahiro.

Wanglong Qiu, Xiaojun Li, Hongyan Tang, Alicia S. Huang, Andrey A

Atypical Protein Kinase C Isoform, aPKCλ, Is Essential for Maintaining Hair Follicle Stem Cell Quiescence Shin-Ichi Osada, Naoko Minematsu, Fumino Oda,

Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34 Rebecca J. Morris, Carl D. Bortner, George.

Th17 Cytokines Stimulate CCL20 Expression in Keratinocytes In Vitro and In Vivo: Implications for Psoriasis Pathogenesis Erin G. Harper, Changsheng Guo,

Selective Ablation of Glucocorticoid Receptor in Mouse Keratinocytes Increases Susceptibility to Skin Tumorigenesis Víctor Latorre, Lisa M. Sevilla,

Ganna Bilousova, Jiang Chen, Dennis R. Roop

Thomas E. Stout, Trevor McFarland, John C

Epidermal Label-Retaining Cells: Background and Recent Applications

Role of the Notch Ligand Delta1 in Embryonic and Adult Mouse Epidermis

Fas and c-kit are Involved in the Control of Hair Follicle Melanocyte Apoptosis and Migration in Chemotherapy-Induced Hair Loss Andrei A. Sharov, Guang-Zhi.

Essential Role of the Keratinocyte-Specific Endonuclease DNase1L2 in the Removal of Nuclear DNA from Hair and Nails Heinz Fischer, Sandra Szabo, Jennifer.

Integrin β6-Deficient Mice Show Enhanced Keratinocyte Proliferation and Retarded Hair Follicle Regression after Depilation Yanshuang Xie, Kevin J. McElwee,

Dickkopf 1 Promotes Regression of Hair Follicles

Transcription Factor CTIP2 Maintains Hair Follicle Stem Cell Pool and Contributes to Altered Expression of LHX2 and NFATC1 Shreya Bhattacharya, Heather.

Mohammad Rashel, Ninche Alston, Soosan Ghazizadeh

Molecular Clocks in Mouse Skin

Reduced Susceptibility to Two-Stage Skin Carcinogenesis in Mice with Epidermis- Specific Deletion of Cd151 Norman Sachs, Pablo Secades, Laura van Hulst,

Fuz Controls the Morphogenesis and Differentiation of Hair Follicles through the Formation of Primary Cilia Daisy Dai, Huiping Zhu, Bogdan Wlodarczyk,

Alexandra Charruyer, Lauren R. Strachan, Lili Yue, Alexandra S

Lack of Collagen VI Promotes Wound-Induced Hair Growth

The Neurofibromatosis Type 1 (Nf1) Tumor Suppressor is a Modifier of Carcinogen- Induced Pigmentation and Papilloma Formation in C57BL/6 Mice Radhika.

Sirolimus Reduces the Incidence and Progression of UVB-Induced Skin Cancer in SKH Mice even with Co-administration of Cyclosporine A Brian C. Wulff,

Ute Breitenbach, Jan P. Tuckermann, Christoffer Gebhardt, Karl H

Extracellular Adherence Protein of Staphylococcus aureus Suppresses Disease by Inhibiting T-Cell Recruitment in a Mouse Model of Psoriasis Honglin Wang,

14-3-3σ Regulates Keratinocyte Proliferation and Differentiation by Modulating Yap1 Cellular Localization Sumitha A.T. Sambandam, Ramesh B. Kasetti,

Different Consequences of β1 Integrin Deletion in Neonatal and Adult Mouse Epidermis Reveal a Context-Dependent Role of Integrins in Regulating Proliferation,

A Simple In Vivo System for Studying Epithelialization, Hair Follicle Formation, and Invasion Using Primary Epidermal Cells from Wild-Type and Transgenic.

Modulation of Hair Growth with Small Molecule Agonists of the Hedgehog Signaling Pathway Rudolph D. Paladini, Jacqueline Saleh, Changgeng Qian, Guang-Xin.

The Vitamin D Receptor Is Required for Mouse Hair Cycle Progression but not for Maintenance of the Epidermal Stem Cell Compartment Héctor G. Pálmer,

Yuko Oda, Lizhi Hu, Vadim Bul, Hashem Elalieh, Janardan K

Thuyen Nguyen, Maria L. Wei Journal of Investigative Dermatology

Roles of Aquaporin-3 in the Epidermis

Transient Expression of Ephrin B2 in Perinatal Skin Is Required for Maintenance of Keratinocyte Homeostasis Gyohei Egawa, Masatake Osawa, Akiyoshi Uemura,

Ephrin-A2 and Ephrin-A5 Are Important for the Functional Development of Cutaneous Innervation in a Mouse Model Dulharie T. Wijeratne, Jennifer Rodger,

Murine Epidermal Label-Retaining Cells Isolated by Flow Cytometry do not Express the Stem Cell Markers CD34, Sca-1, or Flk-1 Michael R. Albert, Ruth-Ann.

Alterations in Cholesterol Sulfate and its Biosynthetic Enzyme During Multistage Carcinogenesis in Mouse Skin Kaoru Kiguchi, John DiGiovanni Journal.

Characterization of Quiescent Epithelial Cells in Mouse Meibomian Glands and Hair Follicle/Sebaceous Glands by Immunofluorescence Tomography Geraint.

Delineating Immune-Mediated Mechanisms Underlying Hair Follicle Destruction in the Mouse Mutant Defolliculated Fiona Ruge, Aikaterini Glavini, Awen M.

An Extended Epidermal Response Heals Cutaneous Wounds in the Absence of a Hair Follicle Stem Cell Contribution Abigail K. Langton, Sarah E. Herrick,

Dihydrotestosterone-Inducible IL-6 Inhibits Elongation of Human Hair Shafts by Suppressing Matrix Cell Proliferation and Promotes Regression of Hair Follicles.

Normal Wound Healing in Mice Deficient for Fibulin-5, an Elastin Binding Protein Essential for Dermal Elastic Fiber Assembly Qian Zheng, Jiwon Choi,

The EGFR Is Required for Proper Innervation to the Skin

Ingenol Mebutate Field-Directed Treatment of UVB-Damaged Skin Reduces Lesion Formation and Removes Mutant p53 Patches Sarah-Jane Cozzi, Steven M. Ogbourne,

Gender Differences in Mouse Skin Morphology and Specific Effects of Sex Steroids and Dehydroepiandrosterone Lamia Azzi, Mohamed El-Alfy, Céline Martel,

Expression of Activated MEK1 in Differentiating Epidermal Cells Is Sufficient to Generate Hyperproliferative and Inflammatory Skin Lesions Robin M. Hobbs,

Urokinase is a Positive Regulator of Epidermal Proliferation In Vivo

Betacellulin Regulates Hair Follicle Development and Hair Cycle Induction and Enhances Angiogenesis in Wounded Skin Marlon R. Schneider, Maria Antsiferova,

Herlina Y. Handoko, Neil F. Box, Graeme J. Walker

C/EBPα Expression Is Downregulated in Human Nonmelanoma Skin Cancers and Inactivation of C/EBPα Confers Susceptibility to UVB-Induced Skin Squamous Cell.

Loss of Keratin 10 Leads to Mitogen-activated Protein Kinase (MAPK) Activation, Increased Keratinocyte Turnover, and Decreased Tumor Formation in Mice

The Requirement for Perp in Postnatal Viability and Epithelial Integrity Reflects an Intrinsic Role in Stratified Epithelia Michelle R. Marques, Rebecca.

Keratinocyte-Derived Granulocyte-Macrophage Colony Stimulating Factor Accelerates Wound Healing: Stimulation of Keratinocyte Proliferation, Granulation.

Mechanism of Sustained Release of Vascular Endothelial Growth Factor in Accelerating Experimental Diabetic Healing Harold Brem, Arber Kodra, Michael.

Presentation transcript:

Bone Morphogenetic Protein 5 Regulates the Number of Keratinocyte Stem Cells from the Skin of Mice Thaned Kangsamaksin, Rebecca J. Morris Journal of Investigative Dermatology Volume 131, Issue 3, Pages 580-585 (March 2011) DOI: 10.1038/jid.2010.378 Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Localization of bone morphogenetic protein 5 (Bmp5) in mouse hair follicles and interfollicular epidermis of wild-type but not mutant mouse skin. Adult mouse skin was collected for paraffin-embedded histological sections for (a) C57BL/6, (b) BALB/c, (c) wild-type, and (d) homozygous short-ear mutant mice at ∼7 to 8 weeks of age, and BMP5 immunohistochemistry was performed. B, hair follicle bulge; D, dermis; E, epidermis; HF, hair follicle; SG, sebaceous gland. Note the intense staining in the hair follicle and in an occasional interfollicular cell of the wild-type epithelium. In the homozygous short-ear mutant skin, Bmp5 staining was consistently absent from hair follicles and interfollicular epidermis. Scale bars=30μm. Journal of Investigative Dermatology 2011 131, 580-585DOI: (10.1038/jid.2010.378) Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Quantification of mouse keratinocyte colonies. Keratinocytes were isolated from 7- to 8-week-old mice. (a) The photographs of the colony assays in 60-mm dishes are shown. The (b) number and (c) size of mouse keratinocyte colonies from all genotypes were quantified after 2 weeks of cultivation on irradiated 3T3 fibroblasts as a feeder layer. Note that the number of colonies is significantly less in the homozygous short-ear mutant mice, whereas colony number and size were relatively greater in the heterozygous and wild-type congenic littermates. Average number of colonies±SD. *P-value <0.003, N=3. Journal of Investigative Dermatology 2011 131, 580-585DOI: (10.1038/jid.2010.378) Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Quantification of label-retaining cells (LRCs) in mouse epidermis. (a–d) Immunohistographs for LRCs are shown. Mice received twice-daily (1000 and 1700hours) subcutaneous injections of 50μg per g body weight of BrdU at postnatal days D3, D4, and D5. After 8–10 weeks, dorsal skins were collected and fixed with 10% formalin for at least 24hours. Cells retaining the label are identified as LRCs. (e) The bar graph shows the quantification of labeled cells in the interfollicular epidermis and hair follicles combined. Labeled cells were counted in the same region of the dorsal skin for every 500 cells. Mean±SD. *P-value <0.05. N=3. Scale bars=30μm. Journal of Investigative Dermatology 2011 131, 580-585DOI: (10.1038/jid.2010.378) Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Exogenous recombinant human bone morphogenetic protein 5 (BMP5) increases keratinocyte colony size and number. (a) To determine the functional significance of Bmp5 on keratinocyte colony formation, we treated wild-type and short-ear keratinocytes cultured on 3T3 feeder layers with different concentrations of recombinant human BMP5 (0.01 and 0.1μgml–1). The recombinant peptide was added to the medium that was changed every other day up to 2 weeks of culture. Note that exogenous BMP5 added to culture increased the number and size of colonies in keratinocytes from all three genotypes. (b) The photographs of the colony assays each correspond to the bar graph directly above. Average number of colonies±SD. *P-value <0.05. N=3. Journal of Investigative Dermatology 2011 131, 580-585DOI: (10.1038/jid.2010.378) Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Bone morphogenetic protein 5 (Bmp5) regulates the number of tumors in mice in the two-stage skin carcinogenesis model. (a) The bar graphs represent the average number of skin tumor across all genotypes. Mouse skin was initiated with 200nmol of 7,12-dimethylbenz[a]anthracene (DMBA) in 200μl of spectral grade acetone when the mice were 7 weeks of age. After 1 week, there was twice-weekly promotion with 17nmol of 12-O-tetradecanoylphorbol-13-acetate (TPA) in 200μl of acetone. Skin tumors were counted weekly for 20 weeks. Note that Bmp5 status was associated with the number of skin tumors, with short-ear mutant and BALB/c mice developing more skin tumors than heterozygous and wild-type littermates, or C57BL/6J wild-type mice. Wild-type C57BL/6 and BALB/c mice were used, respectively, as low- and high- tumor controls. (b) The marked-line chart shows the appearance of tumors across all genotypes. Mean±SD. *P-value <0.05. N=5. Journal of Investigative Dermatology 2011 131, 580-585DOI: (10.1038/jid.2010.378) Copyright © 2011 The Society for Investigative Dermatology, Inc Terms and Conditions