Joint dependent concentrations of bone alkaline phosphatase in serum and synovial fluids of horses with osteochondral injury: an analytical and clinical.

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Joint dependent concentrations of bone alkaline phosphatase in serum and synovial fluids of horses with osteochondral injury: an analytical and clinical validation T.N. Trumble, D.V.M., Ph.D., M.P. Brown, D.V.M., M.Sc., K.A. Merritt, B.S., R.C. Billinghurst, D.V.M., Ph.D. Osteoarthritis and Cartilage Volume 16, Issue 7, Pages 779-786 (July 2008) DOI: 10.1016/j.joca.2007.11.008 Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Validation of BAP immunoassay for use in equine serum and SF. (A) Demonstration of parallelism: absorbance vs concentration of diluted (1:2, 1:4, 1:8, and 1:10) pooled serum and SF samples (squares) is compared with the curve derived from standards provided by the manufacturer (triangles). Coefficient correlations for the samples (black) and standards (gray) are listed next to each corresponding line. (B) Demonstration of linearity: dilutions of serum (solid lines) and SF (dashed lines) samples at 1:2, 1:4, 1:8, and 1:10 for each QCH, QCM, and QCL samples. The concentration of each dilution was determined from the standard curve, and the observed concentration was plotted against the reciprocal of the dilution (1/dilution). Osteoarthritis and Cartilage 2008 16, 779-786DOI: (10.1016/j.joca.2007.11.008) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Scattergram with mean BAP concentrations in (A) serum, (B) SF, and (C) the ratio of SF to serum for normal horses and horses with OC injury of the carpal (triangles) or MP (circles) joints. Significant differences between groups are represented as *P<0.05, **P<0.01, and ***P<0.001. Red lines represent concentrations (<30U/L for serum, >22U/L for carpal SF, and ≥0.5 for carpal SF:serum) for which there is predictive value in determining OC injury from normal. Osteoarthritis and Cartilage 2008 16, 779-786DOI: (10.1016/j.joca.2007.11.008) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions