Rheological changes in human red blood cells under oxidative stress

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Rheological changes in human red blood cells under oxidative stress Iwona Cicha, Yoji Suzuki, Norihiko Tateishi, Nobuji Maeda Pathophysiology Volume 6, Issue 2, Pages 103-110 (July 1999) DOI: 10.1016/S0928-4680(99)00005-X

Fig. 1 Protein content in Fe2+/ascorbate-treated RBCs. (A) Densitometric SDS–PAGE patterns of membrane proteins under reducing conditions. The positions of bands are as indicated; N, new band of 60 kDa; n, new band of 35 kDa. (B) Quantified results: (a) band 3; (b) new band (60 kDa); (c) membrane-bound hemoglobin. Protein bands are expressed as a percentage of the total content of all bands. The values represented by bars are means±S.D. (n=4). *P<0.001; **P<0.01 vs. control, ns, not significant. Pathophysiology 1999 6, 103-110DOI: (10.1016/S0928-4680(99)00005-X)

Fig. 2 The effect of diamide on the densitometric SDS–PAGE patterns of membrane proteins under non-reducing conditions. The positions of bands are as indicated; HMWA, high-molecular weight aggregates. Pathophysiology 1999 6, 103-110DOI: (10.1016/S0928-4680(99)00005-X)

Fig. 3 The effects of different concentrations of oxidants on human RBCs deformability. (a) Fe2+/ascorbate treatment; (b) diamide treatment. Pathophysiology 1999 6, 103-110DOI: (10.1016/S0928-4680(99)00005-X)

Fig. 4 Rouleaux formation rate of oxidatively modified RBCs. (a) Fe2+/ascorbate treatment (n=9); (b) diamide treatment (n=6). Pathophysiology 1999 6, 103-110DOI: (10.1016/S0928-4680(99)00005-X)

Fig. 5 The typical time-courses of RBC aggregation. (a) RBCs treated with 2 mM FeSO4; (b) RBCs treated with 0.5 mM of diamide. Pathophysiology 1999 6, 103-110DOI: (10.1016/S0928-4680(99)00005-X)

Fig. 6 Suspension viscosity of oxidatively modified RBCs at Ht=30%. (a) Fe2+/ascorbate treatment (n=6); (b) diamide treatment (n=14). Pathophysiology 1999 6, 103-110DOI: (10.1016/S0928-4680(99)00005-X)