Biochemical characterization of the protein phosphatases Saci-PP2A.

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Biochemical characterization of the protein phosphatases Saci-PP2A. Biochemical characterization of the protein phosphatases Saci-PP2A. The activity of Saci-PP2A was determined in the presence of pNPP (A), the phospho-Thr peptide RRA(pT)VA (B) as well as in the presence of okadaic acid (p-Thr peptide RRA(pT)VA)(C). All experiments were performed at 70 °C in triplicate. Saci_PP2A revealed phosphatase activity with pNPP as well as the p-Thr peptide, whereas no activity was observed with the p-Tyr peptide. Furthermore, Saci_PP2A was significantly inhibited in presence of okadaic acid, a typical inhibitor of PPPs. Julia Reimann et al. Mol Cell Proteomics 2013;12:3908-3923 © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.