Drug‐induced cell death and proliferation inhibition can be quantified from serial confocal images Drug‐induced cell death and proliferation inhibition.

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Drug‐induced cell death and proliferation inhibition can be quantified from serial confocal images Drug‐induced cell death and proliferation inhibition can be quantified from serial confocal images Schematic overview of drug testing in organoid culture with the DeathPro assay. Cells are grown on Matrigel for 4 days, stained with Hoechst (H) and propidium iodide (PI) and imaged at day 4, day 7 and day 10. Image gallery exemplifies OC12 organoid growth and cell death at start (day 4) and end of carboplatin treatment (day 7) and after carboplatin removal (day 10) using eight carboplatin concentrations or drug‐free medium (ctrl). Confocal images are reduced to maximum intensity projections, and binary images of merged Hoechst (green) and PI (red) channel are shown.Image analysis for the DeathPro assay is based on area measurements in Hoechst and PI channels, and calculation of LD50, AUCd and AUCpi values to describe cell death and growth arrest. Drug response curve fitting and AUC values are illustrated for OC12 at time points depicted in (A).Data information: Grey and orange boxes in (A) correspond to the magnifications in (B). Scale bar is 200 μm. Julia Jabs et al. Mol Syst Biol 2017;13:955 © as stated in the article, figure or figure legend