Weighing in on Adipocyte Precursors Ryan Berry, Elise Jeffery, Matthew S. Rodeheffer  Cell Metabolism  Volume 19, Issue 1, Pages 8-20 (January 2014) DOI: 10.1016/j.cmet.2013.10.003 Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 1 Identification of Mature Adipocyte Nuclei In Situ Images of whole mounted white adipose tissue. The lipid is stained with LipidTOX (green), the plasma membrane with CellMask Orange (red), and the nuclei with DAPI (blue). Most nuclei in WAT are in close proximity to lipid droplets. Therefore, accurate identification of adipocyte nuclei (white arrowheads) from nonadipocyte nuclei (yellow arrowheads) requires staining of the plasma membrane. Nuclei that are not separated from the lipid droplets by a plasma membrane are the mature adipocyte nuclei. Scale bars = 25 μm. Cell Metabolism 2014 19, 8-20DOI: (10.1016/j.cmet.2013.10.003) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 2 A Model of In Vivo Adipogenesis A schematic representation of the adipocyte cellular lineage is shown. The defined lineage, cell surface, and transcriptional markers for each cell type are shown below each listed cell type. Positive markers are shown in blue, while negative markers are shown in gold. The question marks highlight that it is currently unclear if the adipocyte progenitor population contains self-renewing adipocyte stem cells or if the adipocyte progenitors are repopulated by another, unidentified population of adipocyte stem cells in adults. Cell Metabolism 2014 19, 8-20DOI: (10.1016/j.cmet.2013.10.003) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 3 Adipocyte Progenitors in WAT Depots CD24+ adipocyte progenitor cells are a distinct population of WAT-resident stromal cells. Shown are dot plots of the Lin-:CD29+:CD34+ cell populations from the indicated depots. The cells are present in levels as high at 2% in adult SWAT depots. Cells with an identical cell surface marker profile are present in VWAT depots but are less abundant (perigonadal WAT [GWAT] is shown). Cell Metabolism 2014 19, 8-20DOI: (10.1016/j.cmet.2013.10.003) Copyright © 2014 Elsevier Inc. Terms and Conditions