Volume 58, Issue 2, Pages (August 2000)

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Volume 58, Issue 2, Pages 740-747 (August 2000) Renal nitric oxide production during the early phase of experimental diabetes mellitus Shoshana Keynan, Boaz Hirshberg, Nomy Levin-Iaina, Isaiah D. Wexler, Rachel Dahan, Etty Reinhartz, Haim Ovadia, Yoram Wollman, Tamara Chernihovskey, Adrian Iaina, Itamar Raz Kidney International Volume 58, Issue 2, Pages 740-747 (August 2000) DOI: 10.1046/j.1523-1755.2000.00220.x Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 1 Changes in urinary nitric oxide metabolites (uNO2 + NO3) in streptozotocin (STZ) rats during the first week of diabetes. Symbols are: (•) control; (▾) diabetic rats; *P < 0.01; **P < 0.05. Kidney International 2000 58, 740-747DOI: (10.1046/j.1523-1755.2000.00220.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 2 Creatinine clearance in STZ rats during the first week of diabetes. Symbols are: (•) control; (▾) diabetic rats; *P < 0.01 diabetic rats vs. control. Kidney International 2000 58, 740-747DOI: (10.1046/j.1523-1755.2000.00220.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 3 Western blot analysis of liver and kidney cortex homogenate. Fifty micrograms of liver (A) or kidney cortex (B) homogenate was separated on 7.5% SDS-PAGE. After blotting, the membrane was reacted with monoclonal anti-endothelial nitric oxide synthase (anti-eNOS) antibody (1:1000). PC, human endothelial cell lysate. Kidney International 2000 58, 740-747DOI: (10.1046/j.1523-1755.2000.00220.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 4 Effect of five days diabetes on expression of neuronal nitric oxide synthase (nNOS) mRNA in kidney cortex. A representative ethidium bromide-stained agarose gel of kidney cortex nNOS (601 bp) and GAPDH (515 bp) RT-PCR products. Lanes 1 through 3, control rats; lanes 4 through 6, STZ-diabetic rats. Kidney International 2000 58, 740-747DOI: (10.1046/j.1523-1755.2000.00220.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 5 NADPH-diaphorase staining of sections from normal and diabetic rat kidney. (A) Normal rat kidney was prepared and stained as described under the Methods section (magnification ×400). (B) Population of nNOS positive macula densa of the experimental rats. Twelve sections from diabetic (N = 5) and control (N = 5) animals were stained with NADPH-diaphorase. The number of positive macula densa per 100 counted glomeruli is expressed in percentage. *P < 0.01. Kidney International 2000 58, 740-747DOI: (10.1046/j.1523-1755.2000.00220.x) Copyright © 2000 International Society of Nephrology Terms and Conditions

Figure 6 Confocal images of the normal kidney sections immunostained for nNOS. (a) Differential interference contrast (DIC) images were collected simultaneously using a transmitted light detector. (b) A Zeiss LSM 410 confocal laser scanning system attached to the Zeiss Axiovert 135M microscope was utilized Green fluorescence of Alexa-488 (secondary antibody was used at 1:200; Molecular Probes, Eugene, OR, USA) was excited with an argon laser. Kidney International 2000 58, 740-747DOI: (10.1046/j.1523-1755.2000.00220.x) Copyright © 2000 International Society of Nephrology Terms and Conditions