Endocarditis due to Tropheryma whipplei: rapid detection, limited genetic diversity, and long-term clinical outcome in a local experience R. Escher,

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Presentation transcript:

Endocarditis due to Tropheryma whipplei: rapid detection, limited genetic diversity, and long-term clinical outcome in a local experience R. Escher, S. Roth, S. Droz, K. Egli, M. Altwegg, M.G. Täuber Clinical Microbiology and Infection Volume 16, Issue 8, Pages 1213-1222 (August 2010) DOI: 10.1111/j.1469-0691.2009.03038.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Tropheryma whipplei DNA amplicons separated using a 2% agarose gel. Lanes 2–5, negative primer controls without DNA (neg co). Following lanes, DNA from patients 1–4 (PI, P2, P3, P4), with two different heart valve tissue probes of patient 3, amplified with primer pairs HVGS1 (lanes 6–10, 246 bp), HVGS4 (lanes 11–15, 199 bp), whipp (lanes 17–21, 504 bp). M, marker lanes. Clinical Microbiology and Infection 2010 16, 1213-1222DOI: (10.1111/j.1469-0691.2009.03038.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Microscopic examination of the excised valve (squash preparation). Numerous extracellular fine rod-like organisms are visible in an acridine orange stain (arrows; Zeiss Axioplan 2 imaging, filter set 09, × 1000; Carl Zeiss, Oberkochen, Germany). Clinical Microbiology and Infection 2010 16, 1213-1222DOI: (10.1111/j.1469-0691.2009.03038.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions