Volume 54, Issue 6, Pages 2184-2193 (January 1998) Biocompatibility and buffers: Effect of bicarbonate-buffered peritoneal dialysis fluids on peritoneal cell function  Achim Jörres, Thorsten O. Bender, André Finn, Janusz Witowski, Sibylle Fröhlich, Gerhard M. Gahl, Ulrich Frei, Heiko Keck, Jutta Passlick-Deetjen  Kidney International  Volume 54, Issue 6, Pages 2184-2193 (January 1998) DOI: 10.1046/j.1523-1755.1998.00178.x Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 Interleukin-6 release from peritoneal fibroblasts preexposed for up to 90minutes to lactate-buffered, acidic PDF (A) or bicarbonate-buffered, neutral PDF (B) followed by a 24-hour recovery/stimulation period in the presence of IL-1β (100pg/ml). Symbols are: (in A) (□) control; () lactate 1.5%; () lactate 4.25%; (in B) (□) control; () bicarbonate 1.5%; (▪) bicarbonate 4.25%. Data are mean ± sem, N = 6 for lactate-buffered PDF and N = 8 for bicarbonate-buffered PDF. Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 Interleukin-6 release from peritoneal fibroblasts preexposed for up to 90minutes to lactate-buffered, acidic PDF (A) or bicarbonate-buffered, neutral PDF (B) followed by a 24-hour recovery/stimulation period in the presence of IL-1β (100pg/ml). Symbols are: (in A) (□) control; () lactate 1.5%; () lactate 4.25%; (in B) (□) control; () bicarbonate 1.5%; (▪) bicarbonate 4.25%. Data are mean ± sem, N = 6 for lactate-buffered PDF and N = 8 for bicarbonate-buffered PDF. Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 Interleukin-6 release from peritoneal fibroblasts preexposed for up to 90minutes to lactate-buffered, acidic PDF (A) or bicarbonate-buffered, neutral PDF (B) followed by a 24-hour recovery/stimulation period in the presence of IL-1β (100pg/ml). Symbols are: (in A) (□) control; () lactate 1.5%; () lactate 4.25%; (in B) (□) control; () bicarbonate 1.5%; (▪) bicarbonate 4.25%. Data are mean ± sem, N = 6 for lactate-buffered PDF and N = 8 for bicarbonate-buffered PDF. Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 Interleukin-6 release from peritoneal fibroblasts preexposed for up to 90minutes to lactate-buffered, acidic PDF (A) or bicarbonate-buffered, neutral PDF (B) followed by a 24-hour recovery/stimulation period in the presence of IL-1β (100pg/ml). Symbols are: (in A) (□) control; () lactate 1.5%; () lactate 4.25%; (in B) (□) control; () bicarbonate 1.5%; (▪) bicarbonate 4.25%. Data are mean ± sem, N = 6 for lactate-buffered PDF and N = 8 for bicarbonate-buffered PDF. Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 Interleukin-6 release from peritoneal mesothelial cells pre-exposed for up to 60minutes to lactate-buffered acidic PDF (A) or bicarbonate-buffered neutral PDF (B) followed by a 24-hour recovery/stimulation period in the presence of IL-1β (100pg/ml). Symbols are: (in A) (□) control; () lactate 1.5%; () lactate 4.25%; (in B) (□) control; () bicarbonate 1.5%; (▪) bicarbonate 4.25%. Data are mean ± sem, N = 6 for both lactate-buffered and bicarbonate-buffered PDF. *Statistical significance (P < 0.05) versus controls (cell culture medium) at the corresponding time point. Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 Interleukin-6 release from peritoneal mesothelial cells pre-exposed for up to 60minutes to lactate-buffered acidic PDF (A) or bicarbonate-buffered neutral PDF (B) followed by a 24-hour recovery/stimulation period in the presence of IL-1β (100pg/ml). Symbols are: (in A) (□) control; () lactate 1.5%; () lactate 4.25%; (in B) (□) control; () bicarbonate 1.5%; (▪) bicarbonate 4.25%. Data are mean ± sem, N = 6 for both lactate-buffered and bicarbonate-buffered PDF. *Statistical significance (P < 0.05) versus controls (cell culture medium) at the corresponding time point. Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 Interleukin-6 release from peritoneal mesothelial cells pre-exposed for up to 60minutes to lactate-buffered acidic PDF (A) or bicarbonate-buffered neutral PDF (B) followed by a 24-hour recovery/stimulation period in the presence of IL-1β (100pg/ml). Symbols are: (in A) (□) control; () lactate 1.5%; () lactate 4.25%; (in B) (□) control; () bicarbonate 1.5%; (▪) bicarbonate 4.25%. Data are mean ± sem, N = 6 for both lactate-buffered and bicarbonate-buffered PDF. *Statistical significance (P < 0.05) versus controls (cell culture medium) at the corresponding time point. Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 Interleukin-6 release from peritoneal mesothelial cells pre-exposed for up to 60minutes to lactate-buffered acidic PDF (A) or bicarbonate-buffered neutral PDF (B) followed by a 24-hour recovery/stimulation period in the presence of IL-1β (100pg/ml). Symbols are: (in A) (□) control; () lactate 1.5%; () lactate 4.25%; (in B) (□) control; () bicarbonate 1.5%; (▪) bicarbonate 4.25%. Data are mean ± sem, N = 6 for both lactate-buffered and bicarbonate-buffered PDF. *Statistical significance (P < 0.05) versus controls (cell culture medium) at the corresponding time point. Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 3 Interleukin-6 release from IL-1β-stimulated peritoneal fibroblasts coincubated with serial dilutions (vol/vol) of lactate-buffered (A, 1.5% glucose, N = 6; B, 4.25% glucose) or bicarbonate-buffered peritoneal dialysis fluids (C, 1.5% glucose, N = 9; D, 4.25% glucose, N = 7). *Statistical significance (P < 0.05) versus controls (cell culture medium). Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 2184-2193DOI: (10. 1046/j. 1523-1755 Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 2184-2193DOI: (10. 1046/j. 1523-1755 Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 2184-2193DOI: (10. 1046/j. 1523-1755 Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Kidney International 1998 54, 2184-2193DOI: (10. 1046/j. 1523-1755 Kidney International 1998 54, 2184-2193DOI: (10.1046/j.1523-1755.1998.00178.x) Copyright © 1998 International Society of Nephrology Terms and Conditions