Volume 84, Issue 1, Pages (January 2003)

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Volume 84, Issue 1, Pages 326-340 (January 2003) Effect of Hydrophobic Surfactant Proteins SP-B and SP-C on Binary Phospholipid Monolayers Jennifer M. Brockman, Zhengdong Wang, Robert H. Notter, Richard A. Dluhy Biophysical Journal Volume 84, Issue 1, Pages 326-340 (January 2003) DOI: 10.1016/S0006-3495(03)74853-X Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 1 IR external reflection-absorption spectra of a 7:1 DPPC-d62:DPPG+5wt % SP-B/C monolayer film collected at surface pressures from 6.0 to 60.0mN/m. (A) CH2 stretching region between 3000–2800cm−1. (B) CD2 stretching region between 2250–2050cm−1. Biophysical Journal 2003 84, 326-340DOI: (10.1016/S0006-3495(03)74853-X) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 2 IR external reflection-absorption C-H spectral band parameters for 7:1 DPPC-d62: DPPG binary monolayer films with and without SP-B/C added. C-H band parameters reflect changes in structure for the DPPG component in the monolayer. Variations in wavenumber (A) and band height (B) for the CH2 antisymmetric stretching band are shown plotted as a function of surface pressure. See key in A for symbols. Biophysical Journal 2003 84, 326-340DOI: (10.1016/S0006-3495(03)74853-X) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 3 IR external reflection-absorption C-D spectral band parameters for a 7:1 DPPC-d62:DPPG binary monolayer films with and without SP-B/C added. C-D band parameters reflect changes in structure for the DPPC-d62 component in the monolayer. Variations in wavenumber (A) and band height (B) for the CD2 antisymmetric stretching band are shown plotted as a function of surface pressure. See key in A for symbols. Biophysical Journal 2003 84, 326-340DOI: (10.1016/S0006-3495(03)74853-X) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 4 IR external reflection-absorption spectral band parameters for a 7:1 DPPC-d62:DPPG binary monolayer films with and without SP-B/C added. Variation in band height ratio for the CH2/CD2 antisymmetric stretching bands is shown plotted as a function of surface pressure. Changes in C-H/C-D band height ratio indicate relative changes in the DPPG versus DPPC-d62 components. See key in A for symbols. Biophysical Journal 2003 84, 326-340DOI: (10.1016/S0006-3495(03)74853-X) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 5 IR external reflection-absorption C-H and C-D spectral band parameters for 1:1 DPPC-d62:DPPG binary monolayer films with and without SP-B/C added. C-H band parameters reflect changes in structure for the DPPG component in the monolayer whereas C-D band parameters reflect changes in structure for the DPPC-d62 component. Variation in wavenumber for the CH2 (A) and CD2 (B) antisymmetric stretching bands is shown plotted as a function of surface pressure. See key in A for symbols. Biophysical Journal 2003 84, 326-340DOI: (10.1016/S0006-3495(03)74853-X) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 6 IR external reflection-absorption spectral band parameters for (A) 1:1 DPPC-d62:DPPG and (B) 2:1 DPPC-d62:DPPG binary monolayer films with and without SP-B/C added. Variation in band height ratio for the CH2/CD2 antisymmetric stretching bands is shown plotted as a function of surface pressure. Changes in C-H/C-D band height ratio indicate relative changes in the DPPG versus DPPC-d62 components. See key in A for symbols. Biophysical Journal 2003 84, 326-340DOI: (10.1016/S0006-3495(03)74853-X) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 7 IR external reflection-absorption C-H spectral band parameters for a 7:1 DPPC-d62:DOPG binary monolayer films with and without SP-B/C added. C-H band parameters reflect changes in structure for the DOPG component in the monolayer. Variations in wavenumber (A) and band height (B) for the CH2 antisymmetric stretching band are shown plotted as a function of surface pressure. See key in A for symbols. Biophysical Journal 2003 84, 326-340DOI: (10.1016/S0006-3495(03)74853-X) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 8 IR external reflection-absorption C-D spectral band parameters for a 7:1 DPPC-d62:DOPG binary monolayer films with and without SP-B/C added. C-D band parameters reflect changes in structure for the DPPC-d62 component in the monolayer. Variations in wavenumber (A) and band height (B) for the CD2 antisymmetric stretching band are shown plotted as a function of surface pressure. See key in A for symbols. Biophysical Journal 2003 84, 326-340DOI: (10.1016/S0006-3495(03)74853-X) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 9 IR external reflection-absorption spectra band parameters for a 7:1 DPPC-d62:DOPG binary monolayer films with and without SP-B/C added. Variation in band height ratio for the CH2/CD2 antisymmetric stretching bands is shown plotted as a function of surface pressure. Changes in C-H/C-D band height ratio indicate relative changes in the DPPG versus DPPC-d62 components. See key in A for symbols. Biophysical Journal 2003 84, 326-340DOI: (10.1016/S0006-3495(03)74853-X) Copyright © 2003 The Biophysical Society Terms and Conditions