Hong Wu, Mitchell Smith, Michael M

Slides:

Advertisements

Similar presentations

Grover disease with a contagious twist

Advertisements

Large-Cell Transformation of Mycosis Fungoides Occurring at the Site of Previously Treated Cutaneous B-Cell Lymphoma Jennifer L. Herrmann, Naveed Sami

Primary cutaneous B-cell lymphomas with large cell predominance–primary cutaneous follicle center lymphoma, diffuse large B-cell lymphoma, leg type and.

Chemokine Receptor Expression on Neoplastic and Reactive T Cells in the Skin at Different Stages of Mycosis Fungoides Tilmann Kallinich, J. Marcus Muche,

Refractory cutaneous lichenoid sarcoidosis treated with tranilast

Volume 1, Issue 4, Pages (July 2015)

Pathologic Diagnosis of Cutaneous Lymphomas

Josh Wharton, MD, David Roffwarg, BS, Jeremiah Miller, MD, Daniel J

Volume 3, Issue 4, Pages (July 2017)

Gregory D. Rak, Lisa C. Osborne, Mark C. Siracusa, Brian S

Upregulation of Inflammatory Cytokines and Oncogenic Signal Pathways Preceding Tumor Formation in a Murine Model of T-Cell Lymphoma in Skin Xuesong Wu,

Cynthia M. Magro, MD, Tracy L. Davis, MD, PhD, Drew J.B. Kurtzman, MD

Hydroa Vacciniforme Is Associated with Increased Numbers of Epstein–Barr Virus– Infected γδT Cells Yoji Hirai, Takenobu Yamamoto, Hiroshi Kimura, Yoshinori.

Development of a reticular rash in a febrile woman: An unusual cutaneous presentation of angioimmunoblastic T-cell lymphoma Michelle S. Min, MSci, Jonathan.

WHO-EORTC classification for cutaneous lymphomas

Jonathan E. Blume, MD, Howard L. Stoll, MD, Richard T. Cheney, MD

Targeted Overexpression of the Angiogenesis Inhibitor Thrombospondin-1 in the Epidermis of Transgenic Mice Prevents Ultraviolet-B-Induced Angiogenesis.

A definitive case of metastatic folliculotropic melanoma

Cutaneous Drug Delivery: An Update

Liisa Väkevä, Annamari Ranki Journal of Investigative Dermatology

Raymond Barnhill, Katrina Dy, Claire Lugassy

Volume 3, Issue 3, Pages (May 2017)

Rapid onset of CD8+ aggressive T-cell lymphoma during bexarotene therapy in a patient with Sézary syndrome Alexander Kreuter, MD, Peter Altmeyer, MD

Was Treatment the Trigger? Mycosis Fungoides

Massive Recruitment of Type I Interferon Producing Plasmacytoid Dendritic Cells in Varicella Skin Lesions Gianni Gerlini, Giulia Mariotti, Beatrice Bianchi,

Volume 1, Issue 4, Pages (July 2015)

Cutaneous T-Cell Lymphoma: The Yin and Yang of Inflammation and Neoplasia Xuesong Wu, Sam T. Hwang Journal of Investigative Dermatology Symposium Proceedings

The Complexity of Elastic Fiber Biogenesis: The Paradigm of Cutis Laxa

Circulating Tumor Cells and Melanoma Progression

Absence of Microsatellite Instability and Lack of Evidence for Subclone Diversification in the Pathogenesis and Progression of Mycosis Fungoides Chalid.

Reed syndrome presenting with leiomyosarcoma

The Molecular Basis of Lymphocyte Recruitment to the Skin: Clues for Pathogenesis and Selective Therapies of Inflammatory Disorders Michael P. Schön,

Francois le Pelletier, Anne Janin Journal of Investigative Dermatology

Identification of a Novel CD160+CD4+ T-Lymphocyte Subset in the Skin: A Possible Role for CD160 in Skin Inflammation Sofia Abecassis, Jérôme Giustiniani,

Dermal Dendritic Cells Associated with T Lymphocytes in Normal Human Skin Display an Activated Phenotype Alexander D. McLellan, Axel Heiser, Rüdiger.

Clinical Snippets Journal of Investigative Dermatology

Minutes of the Board of Directors Meeting

Human Dendritic Cells as Targets of Dengue Virus Infection

Inflammatory Events Are Involved in Acne Lesion Initiation

Superantigens, Autoantigens, and Pathogenic T Cells in Psoriasis

Murine Epidermal Label-Retaining Cells Isolated by Flow Cytometry do not Express the Stem Cell Markers CD34, Sca-1, or Flk-1 Michael R. Albert, Ruth-Ann.

TCRγ-Chain Gene Rearrangement by PCR-Based GeneScan: Diagnostic Accuracy Improvement and Clonal Heterogeneity Analysis in Multiple Cutaneous T-Cell Lymphoma.

Volume 5, Issue 2, Pages (February 2019)

Botulinum Neurotoxin A Decreases Infiltrating Cutaneous Lymphocytes and Improves Acanthosis in the KC-Tie2 Mouse Model Nicole L. Ward, Kevin D. Kavlick,

Journal of Investigative Dermatology

Journal of Investigative Dermatology

Large-Cell Transformation of Mycosis Fungoides Occurring at the Site of Previously Treated Cutaneous B-Cell Lymphoma Jennifer L. Herrmann, Naveed Sami

Ultraviolet B-Induced Skin Angiogenesis Is Associated with a Switch in the Balance of Vascular Endothelial Growth Factor and Thrombospondin-1 Expression

Primary Cutaneous Follicle Center Cell Lymphomas and Large B Cell Lymphomas of the Leg Descend from Germinal Center Cells. A Single Cell Polymerase Chain.

Society for Investigative Dermatology 2010 Meeting Minutes

CXCL12 as a Predictor of Vitiligo Activity and Disease Progression

Expression of the CD4+ Cell-Specific Chemoattractant Interleukin-16 in Mycosis Fungoides Volker Blaschke, Kristian Reich, Michael Letschert, Florian.

Myelodysplasia Cutis Versus Leukemia Cutis

Research Snippets Journal of Investigative Dermatology

MYD88 Somatic Mutation Is a Genetic Feature of Primary Cutaneous Diffuse Large B- Cell Lymphoma, Leg Type Anne Pham-Ledard, David Cappellen, Fabian Martinez,

Expression of Mast Cell Growth Modulating and Chemotactic Factors and their Receptors in Human Cutaneous Scars Barbara Hermes Journal of Investigative.

Research Snippets from the British Journal of Dermatology

T Cell Receptor-γ Gene Analysis of CD30+ Large Atypical Individual Cells in CD30+ Large Primary Cutaneous T Cell Lymphomas Sylke Gellrich, Anke Wilks,

Self-limited variant of febrile ulceronecrotic Mucha-Habermann disease with polyclonal T-cell receptor rearrangement Peter Helmbold, Gabriele Gaisbauer,

Journal of Investigative Dermatology

Decrease of Skin Infiltrating and Circulating CCR10+ T Cells Coincides with Clinical Improvement after Topical Tacrolimus in Omenn Syndrome Claudia M.

Emilie Doré Badje, MD, Trilok Tejasvi, MD, Alexandra Hristov, MD

Pigmented mammary Paget disease positive for melanocytic markers

Lack of Membrane Expression of Interleukin-2 Receptor α Chain (CD25) in Mycosis Fungoides: Application of Laser Scanning Cytometry for Phenotyping of.

Journal of Investigative Dermatology

CD4/CD8 double-negative early-stage mycosis fungoides associated with primary cutaneous follicular center lymphoma Jeong Hee Cho-Vega, MD, PhD, Jaime.

Variable CD7 Expression on T Cells in the Leukemic Phase of Cutaneous T Cell Lymphoma (Sézary Syndrome) Eric C. Vonderheid, Amy Kotecha, Christine M.

Transforming growth factor–β overexpression in cutaneous extramedullary hematopoiesis of a patient with myelodysplastic syndrome associated with myelofibrosis

Rare presentation of disseminated follicular lymphoma as an ill-defined reticular patch over the scalp and forehead Faezeh Talebi-Liasi, MD, Sophia Sandhu,

Volume 5, Issue 7, Pages (July 2019)

Presentation transcript:

Contribution of Flow Cytometry in the Diagnosis of Cutaneous Lymphoid Lesions Hong Wu, Mitchell Smith, Michael M. Millenson, Nicos Nicolaou, Vivianna M. Van Deerlin, Kathkali Addya, Stuart Lessin, Tahseen Al- saleem Journal of Investigative Dermatology Volume 121, Issue 6, Pages 1522-1530 (December 2003) DOI: 10.1046/j.1523-1747.2003.12631.x Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Multiple erythematous nodules and papules were present on the upper extremities (case 6). Journal of Investigative Dermatology 2003 121, 1522-1530DOI: (10.1046/j.1523-1747.2003.12631.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Cutaneous follicular large cell lymphoma. In case 3, a punch biopsy showed a dense dermal lymphocytic infiltrate with nodular arrangement (a). The infiltrate was composed of large atypical cells (b) that were CD20 positive on an IHC stain (c). Flow cytometry revealed an abnormal population of B cells with dim λ light chain expression (d, R2 indicated by red). When the large cells were gated for analysis (e), there was a predominance of λ-expressing cells (f, R2 indicted by red). Scale bars: (a) 200 μm; (b), (c) 10 μm. Journal of Investigative Dermatology 2003 121, 1522-1530DOI: (10.1046/j.1523-1747.2003.12631.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Cutaneous marginal zone lymphoma. A punch biopsy (case 8) showed patches of lymphocytic infiltrate (a). The cells were medium sized and monotonous (b). Most of the cells were CD20 positive on an IHC stain (c). Flow cytometry analysis gated on the small and medium sized lymphocytes (d) showed a striking predominance of κ positive CD19+ B cells (e, f). Scale bars: (a) 200 μm; (b), (c) 10 μm. Journal of Investigative Dermatology 2003 121, 1522-1530DOI: (10.1046/j.1523-1747.2003.12631.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Cutaneous lymphoma with crush artifact on histologic sections. A punch biopsy (case 4) showed a lymphocytic infiltrate in the dermis, with crush artifacts in many areas (a). In some areas, there appeared to be an increased number of large, atypical cells (b). A CD20 immunostain showed increased numbers of CD20+ B cells (c). Flow cytometry analysis on the gated large lymphoid cells (d) revealed a predominance of κ-expressing CD19+ B cells over λ-expressing B cells (e, f). Scale bars: (a) 100 μm; (b), (c) 10 μm. Journal of Investigative Dermatology 2003 121, 1522-1530DOI: (10.1046/j.1523-1747.2003.12631.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Tumor stage MF with large cell transformation. A punch biopsy (case 19) showed a dermal tumor (a). In addition to the numerous small to medium sized atypical lymphocytes, there were increased numbers of large atypical cells and increased mitoses (b). Flow cytometry revealed an abnormal T cell population that was CD3+(dim), CD4+, and lacked CD7 expression (c, d, indicated in red). Scale bars: (a) 200 μm; (b) 10 μm. Journal of Investigative Dermatology 2003 121, 1522-1530DOI: (10.1046/j.1523-1747.2003.12631.x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions