Expression of EXP1-BirA

Slides:

Advertisements

Similar presentations

Michael T. Jacobsen, Michael Fairhead, Per Fogelstrand, Mark Howarth

Advertisements

by Hee-Don Chae, Katherine E. Lee, David A. Williams, and Yi Gu

Dynamics of ookinete to oocyst transition, and effects of SRPN2 knockdown. Dynamics of ookinete to oocyst transition, and effects of SRPN2 knockdown. Mosquitoes.

Differential proteome analysis of normal and osteoarthritic chondrocytes reveals distortion of vimentin network in osteoarthritis S. Lambrecht, M.Pharm.,

Peixoto et al, Supplementary Figure 4.1 A(top)

Volume 66, Issue 1, Pages (July 2004)

The ΔrlmA mutant strain has impaired CWI pathway activation.

UUKV NSs interacts with MAVS

Volume 15, Issue 8, Pages (May 2016)

Volume 23, Issue 3, Pages (February 2013)

Elif Nur Firat-Karalar, Navin Rauniyar, John R. Yates, Tim Stearns

Alveolar lymphangiogenesis is a feature of idiopathic pulmonary fibrosis (IPF). a) Tissue sections reacted with anti-D2-40 (brown) and anti-CD34 (red)

HRTV and SFTSV NSs, but not UUKV NSs, inhibits JAK/STAT IFN signaling.

Candidates with a PVM localization.

Jungmook Lyu, Vicky Yamamoto, Wange Lu Developmental Cell

Improved design and localization of mtZFNs Schematic structure of previous mtZFN architecture. Improved design and localization of mtZFNs Schematic structure.

EPS15L1 is predominantly expressed in neurons and surviving Eps15L1-KO mice have development and neural deficits. EPS15L1 is predominantly expressed in.

Ras1 is a specific target of Ram1.

KIF1Bβ promotes DHX9 nuclear localization.

Volume 12, Issue 6, Pages (December 2012)

Subcellular localization of UUKV, HRTV, and SFTSV NSs

MET inhibition promotes p21 nuclear translocation

Apoptosis and necroptosis of L929 cells results in the release of mitochondria. Apoptosis and necroptosis of L929 cells results in the release of mitochondria.

Volume 16, Issue 6, Pages (December 2014)

Binding competition between B2′B3 and B3 fragments of TcdB.

SUMO-1 Modification Represses Sp3 Transcriptional Activation and Modulates Its Subnuclear Localization Sarah Ross, Jennifer L Best, Leonard I Zon, Grace.

ZBTB48 is required for MTFP1 expression

Three different mimotope peptides mimic different domains of the the P berghei GAPDH molecule. Three different mimotope peptides mimic different domains.

Tagging of the endogenous Py03652 gene with the gfp gene in Plasmodium yoelii. Tagging of the endogenous Py03652 gene with the gfp gene in Plasmodium yoelii.

PVM candidates with a signal peptide and/or at least one transmembrane domain. PVM candidates with a signal peptide and/or at least one transmembrane domain.

Localization of PBANKA_ and PBANKA_ during blood and liver stage development. Localization of PBANKA_ and PBANKA_ during blood.

ILK knockdown decreases mTOR signaling in PKD kidneys.

Establishment of a TgGAMA conditional-knockdown strain.

Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7 cells or RPE cells. Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7.

ACF can be tethered to a reporter locus through GAL4-DBD.

The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin.

Volume 12, Issue 9, Pages (September 2005)

FGF/ERK inhibition accelerates neural fate conversion of EpiSCs.

PfSec13 does not co-localize with P. falciparum heterochromatin.

Apical localization of PKA fusion constructs in infected and fully differentiated human airway epithelial cells. Apical localization of PKA fusion constructs.

High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. High-affinity binding to the.

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

Inflammasome is not activated in stable chronic obstructive pulmonary disease (COPD). a) Caspase-1 protein levels measured by ELISA and relative mRNA expression.

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

Dissociation of HP1α from H3K9me3 is critical to potentiation of steady-state ATM activity. Dissociation of HP1α from H3K9me3 is critical to potentiation.

Fgfr3;4 mutant lungs display an increase in Mfap5, Igf1 and Fbn2 expression. Fgfr3;4 mutant lungs display an increase in Mfap5,Igf1and Fbn2 expression.

IGF-II and IGFBP-5 rescue the atrophy of myotubes induced by adipocytes. IGF-II and IGFBP-5 rescue the atrophy of myotubes induced by adipocytes. A–D:

Stability of SsbA (A) and GFP (B) proteins in UA159 and ΔclpX strains.

Domain organization of GAMA proteins and proteolytic processing of TgGAMA. Domain organization of GAMA proteins and proteolytic processing of TgGAMA. (A)

Integrin β3 is dispensable for RhoA activity and contractility.

EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed cells. EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed.

Histone crotonylation in kidney tubular cells.

Volume 9, Issue 1, Pages (January 2002)

Subnuclear localization of HA-tagged EBNA1 protein.

TbERP1, TbERP2, TbERP4, and TbERP8 localize to ERES in PCF cells.

PTZ-induced c-Fos and Arc proteins in hippocampal dentate gyrus

IR expression in the olfactory epithelium.

CIN85 interacts with PHD2. CIN85 interacts with PHD2. A, Endogenous PHD2 was immunoprecipitated (IP) with CIN85 antibody from MDA-MB-231, BT-549, and Hs.

CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection.

Fig. 8 C9orf72 knockdown results in an increase in autophagic flux.

Identification of PVM candidates by quantitative mass spectrometry.

Fig. 3 C9ORF72 interacts with SMCR8 in a DENN domain–dependent manner.

Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. Cellular localization of Panx1 and altered.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

GlcN-induced knockdown of PfHsp70x does not affect intraerythrocytic growth. GlcN-induced knockdown of PfHsp70x does not affect intraerythrocytic growth.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

Fig. 2 hTERT induces expression of heat shock protein genes through HSF1 and interacts with Hsp70-1. hTERT induces expression of heat shock protein genes.

Fig. 5 hTERT promotion of Hsp70-1 telomere localization is dependent on Apollo and assists the telomere protective function of Apollo. hTERT promotion.

PfHsp70x knockout does not inhibit export of PfEMP1 to the host cell.

Primary GBM cells are permissive to HCMV in vitro.

Presentation transcript:

Expression of EXP1-BirA Expression of EXP1-BirA*-V5 leads to biotinylation of proteins at the parasite periphery. Expression of EXP1-BirA*-V5 leads to biotinylation of proteins at the parasite periphery. (A) Localization of EXP1 within Plasmodium-infected erythrocytes. (B) Schematic representation of the expression cassette encoding EXP1 fused to BirA* with a C-terminal V5 tag driven by the constitutive eef1α promoter. (C) IFA of EXP1-BirA*-V5 blood stage parasites grown for 18 h in the presence of biotin. Biotinylated proteins were detected by Alexa Fluor 488-labeled streptavidin (Strep; green). The cytosolic mCherry is shown in red and the V5 signal in purple. Parasite nuclei were stained with DAPI and are displayed in blue. Scale bar, 2 μm. (D) Western blot analysis of lysates from EXP1-BirA*-V5 parasites grown for 18 h with or without biotin and WT parasites grown in the presence of biotin. Lysates were probed with fluorescently labeled streptavidin to detect biotinylated proteins. The EXP1-BirA*-V5 fusion protein is predicted to be 55 kDa (asterisk). Cytosolic mCherry detected with an anti-RFP antibody served as a loading control. Cilly Bernardette Schnider et al. mSphere 2018; doi:10.1128/mSphere.00522-17