Biology of Blood and Marrow Transplantation G-CSF Treatment Further Impairs T-Cell Reconstitution in Patients with Residual Anti- Thymocyte Globulin Exposure after Hematopoietic Cell Transplantation: Implications for G-CSF Use?  Coco de Koning, Julie-Anne Gabelich, Jurgen Langenhorst, Rick Admiraal, Jurgen Kuball, Stefan Nierkens, Jaap-Jan Boelens  Biology of Blood and Marrow Transplantation  Volume 24, Issue 3, (March 2018) DOI: 10.1016/j.bbmt.2017.12.589 Copyright © 2017 Terms and Conditions

Figure 1 The effect of ATG exposure on immune recovery in patints after HCT. Residual ATG exposure affects T-cell recovery ( defined as ≥50*106 CD4+ T-cells/L in 2 consecutive measurements after HCT) higher in CB recipients (n = 155, p = 0.002) compared to BM/PB recipients (n = 120, P = 0.68): P values are for the comparison between all four groups (log-rank test). Patients with a follow-up time of less than 100 days were excluded from analysis. T-cell IR was faster after CBT compared to BMT (P = .037). Biology of Blood and Marrow Transplantation 2018 24, DOI: (10.1016/j.bbmt.2017.12.589) Copyright © 2017 Terms and Conditions

Figure 2 Higher ATG-mediated cytotoxicity by neutrophils after in vivo G-CSF treatment. (A-B) difference between ATG-mediated neutrophil target-cell killing from donors receiving (dark grey) or not receiving G-CSF (ligh grey). (C) expression of degranulation and activation markers on neutrophils. (D) confocal images from neutrophils (red) phagocytizing target-cells (blue) only when treated with G-CSF, in presence of ATG. Biology of Blood and Marrow Transplantation 2018 24, DOI: (10.1016/j.bbmt.2017.12.589) Copyright © 2017 Terms and Conditions