Complete nucleotide sequence analysis of the VP1 genomic region of Echoviruses 6 isolated from sewage in Greece revealed 98% similarity with Echoviruses.

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Complete nucleotide sequence analysis of the VP1 genomic region of Echoviruses 6 isolated from sewage in Greece revealed 98% similarity with Echoviruses 6 that were characterized from an aseptic meningitis outbreak 1 year later Z. Kyriakopoulou, E. Dedepsidis, V. Pliaka, D. Tsakogiannis, I.G.A. Ruether, A. Krikelis, P. Markoulatos Clinical Microbiology and Infection Volume 17, Issue 8, Pages 1170-1173 (August 2011) DOI: 10.1111/j.1469-0691.2010.03381.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Phylogenetic tree of 358nt (2446–3307nt) of a region of VP1 of LR51A5 and LR61G3 and other E6 strains: D’ Amori (AY302558), Lytic strain (U16283), Cox (AF081322), Sego (DQ345772), Constant (DQ345771), 25214_Aze_05 (EF397658), 26345_Khab_06 (EF397654), 26082_Ukr_06 (EF397650), 25308_GEO_05 (EF397643), 25214_Tumen_05 (EF397641), 25781_Tumen_2006 (EF397646), 25465_Tambov_05 (EF397644), 25868_Kgz_06 (EF397647), 143–98 (AB268168), RO-28/12/79 (AJ279162), Germany/120/2003 (AY956571), CF219087-06 (AM711095), EV6-14103-00 (AY896761), EV6-10887-99 (AY896760), 87CF36eu (AJ241437), Ver (AY697453), Paran (AY697448), Tsikan (AY697451), Kras (AY697456), ECV6/51.07/2007/GRC (FM878835), and ECV6/82.07/2007/GRC (FM878836). The year of isolation of each strain is presented in parentheses. The construction of the phylogenetic tree was based on a neighbour-joining (NJ) algorithm. Support for specific tree topologies was estimated by bootstrap analysis with 100 pseudoreplicate datasets. Clinical Microbiology and Infection 2011 17, 1170-1173DOI: (10.1111/j.1469-0691.2010.03381.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 (a) SimPlot and (b) Bootscan analysis of the VP1 region of the LR51A5 isolate, created by SimPlot software (version 3.5.1), with a window size of 140nt. Clinical Microbiology and Infection 2011 17, 1170-1173DOI: (10.1111/j.1469-0691.2010.03381.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions