Analysis of a tryptic digest of subunit B12 by MALDI-TOF mass spectrometry. Analysis of a tryptic digest of subunit B12 by MALDI-TOF mass spectrometry.

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Analysis of a tryptic digest of subunit B12 by MALDI-TOF mass spectrometry. Analysis of a tryptic digest of subunit B12 by MALDI-TOF mass spectrometry. The sample was produced by in-gel digestion. The spectrum was calibrated with the ions at m/z 1060.048, 2163.067, and 2273.122. The region between m/z 1250 and 1350 is magnified in the inset. T1 denotes ions of N-terminal peptides from subunit B12. T4 and T9 are ions from the fourth and ninth tryptic peptides from the N terminus of its sequence. The T9-ox ion comes from a version of peptide T9 containing an oxidized methionine residue. Ac-T1 is an ion from the N-α-acetylated form of peptide T1. The numbers +14, +28, and +42 denote ions from peptides with one, two, or three methyl groups attached to histidine residues, respectively. Joe Carroll et al. Mol Cell Proteomics 2005;4:693-699 The American Society for Biochemistry and Molecular Biology