The PbGAPDH G6 fragment interacts with recombinant CD68.

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The PbGAPDH G6 fragment interacts with recombinant CD68. The PbGAPDH G6 fragment interacts with recombinant CD68. Either control human embryonic kidney cells (Cont-293T) or cells transfected to express rat CD68 protein on their surface (CD68-293T) were used. (A) Western blotting confirms CD68 expression by the recombinant, but not control cells. (B) Immunofluorescence assays with non-permeabilized cells show that the CD68 protein is on the surface of the recombinant cells. (C) Flow cytometry assays confirmed CD68 surface expression. (D) Each PbGAPDH sub-cloned fragment (c.f., Fig 2A) was incubated either with CD68-expressing 293T cells or untransfected control cells and analyzed by flow cytometry. PbGAPDH fragment binding was determined with an anti-His tag antibody. Only the G6 fragment detectably bound to the CD68 on the surface of recombinant cells. (E) Nickel-coated ELISA wells were incubated with individual PbGAPDH fragments (Fig 2A), and after triple washes, further incubated with a CD68-293T cell lysate. Recombinant CD68 binding to each sub-cloned PbGAPDH fragment was determined with anti-CD68 antibody. Binding of CD68 to the G6-coated well was significantly stronger than to the pET control. P value was calculated using one-way ANOVA test (**P < 0.01). (F) Either the G6 fragment, the G3 fragment, or pET was incubated with a CD68-293T cell lysate, and the protein that bound to the CD68 was pulled down with an anti-CD68 antibody linked to protein-A agarose. Western blotting of the boiled-eluates determined that G6 bound more strongly to CD68 than G3. All data in this figure are representative of two or more independent experiments. Source data are available for this figure. Sung-Jae Cha et al. LSA 2018;1:e201800111 © 2018 Cha et al.