Filling a GAP—An Optimized Probe for ER Ca2+ Imaging In Vivo Roland Malli, Emrah Eroglu, Markus Waldeck-Weiermair, Wolfgang F. Graier  Cell Chemical Biology  Volume 23, Issue 6, Pages 641-643 (June 2016) DOI: 10.1016/j.chembiol.2016.06.003 Copyright © 2016 Elsevier Ltd Terms and Conditions

Figure 1 Putatively Distinct Sensing Mechanisms of Simple Genetically Encoded Fluorescent Probes The probes consist of a sensor domain (gray/brown structure) and a FP (green barrel). (A) Binding of the analyte (blue circle representing an ion or a small molecule) to the sensor domain induces a conformational distortion, which affects fluorescence of the adjacent FP. Red line indicates the transfer of the conformational change to the FP. (B) The analyte binds in a close position to the FP barrel structure and interferes directly with the chromophore. Red waves indicate direct interaction between the analyte and the FP chromophore. Cell Chemical Biology 2016 23, 641-643DOI: (10.1016/j.chembiol.2016.06.003) Copyright © 2016 Elsevier Ltd Terms and Conditions