Schematic representation of the HAV genome organization, translation products, and regions used for amplification. Schematic representation of the HAV.

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Schematic representation of the HAV genome organization, translation products, and regions used for amplification. Schematic representation of the HAV genome organization, translation products, and regions used for amplification. The area encoding the polyprotein is represented by solid box and the proposed cleavage sites by vertical lines. Regions commonly used for PCR amplifications are as follows: region 1, C terminus of VP3 region (nt 2,020 to nt 2,226); region 2, N terminus of VP1 region (nt 2,172 to nt 2,415); region 3, VP1/P2A junction region (nt 2,984 to nt 3,217); region 4, VP1-P2B region (nt 2,896 to nt 3,289); region 5, entire VP1 region (nt 2,172 to nt 3,125); and region 6, VP3-P2B region (nt 2,133 to nt 3,289). Nucleotide position numbering is according to the HM175 sequence (46). Omana V. Nainan et al. Clin. Microbiol. Rev. 2006; doi:10.1128/CMR.19.1.63-79.2006