Impact of immunotherapy on blood dendritic cells in patients with Hymenoptera venom allergy Katharina Dreschler, Kai Bratke, PhD, Sebastian Petermann,

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Presentation transcript:

Impact of immunotherapy on blood dendritic cells in patients with Hymenoptera venom allergy Katharina Dreschler, Kai Bratke, PhD, Sebastian Petermann, MD, Andrea Bier, MD, Petra Thamm, Michael Kuepper, PhD, J. Christian Virchow, MD, Marek Lommatzsch, MD Journal of Allergy and Clinical Immunology Volume 127, Issue 2, Pages 487-494.e3 (February 2011) DOI: 10.1016/j.jaci.2010.12.003 Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Study design. Measurement of laboratory parameters and analysis of blood DC numbers and characteristics were performed directly before initiation of SIT and 52 hours and 12 months after initiation of SIT. Journal of Allergy and Clinical Immunology 2011 127, 487-494.e3DOI: (10.1016/j.jaci.2010.12.003) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Gating strategy. Total cells in peripheral blood were identified in a forward scatter/side scatter (FSC/SSC) plot (first row). Linneg/dim cells were further gated (second row). Among linneg/dim cells, pDCs were identified by CD123 and HLA-DR expression (third row), mDCs by CD11c and HLA-DR expression (fourth row). Lin, Lineage; PE, phycoerythrin; PerCP, peridinin chlorophyll protein. Journal of Allergy and Clinical Immunology 2011 127, 487-494.e3DOI: (10.1016/j.jaci.2010.12.003) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Peripheral blood pDCs. Shown are the numbers of pDCs in peripheral blood (103/mL) in controls (open circles) and in patients with Hymenoptera venom allergy (closed circles) directly before initiation of SIT and 52 hours and 12 months after initiation of SIT. Each circle represents 1 participant. Median values are shown as red bars. Journal of Allergy and Clinical Immunology 2011 127, 487-494.e3DOI: (10.1016/j.jaci.2010.12.003) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Peripheral blood mDCs. Shown are the numbers of mDCs in peripheral blood (103/mL) in controls (open circles) and in patients with Hymenoptera venom allergy (closed circles) directly before initiation of SIT and 52 hours and 12 months after initiation of SIT. Each circle represents 1 participant. Median values are shown as red bars. Journal of Allergy and Clinical Immunology 2011 127, 487-494.e3DOI: (10.1016/j.jaci.2010.12.003) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Function-associated surface molecules on DCs. Shown are the percentages of peripheral blood pDCs expressing CD40 and peripheral blood mDCs expressing CD40, CD32, and TLR2 in controls (open boxes) and in patients with Hymenoptera venom allergy (gray boxes) directly before initiation of SIT and 52 hours and 12 months after initiation of SIT. Box plots display the median (line within the box), interquartile range (edges of the box), and extremes (vertical lines). Outliers (all cases more distant than 1.5 interquartile ranges from the upper or lower quartile) were omitted in the graphs. Journal of Allergy and Clinical Immunology 2011 127, 487-494.e3DOI: (10.1016/j.jaci.2010.12.003) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Expression of surface molecules on pDCs Expression of surface molecules on pDCs. Histograms display the expression of surface and intracellular (+) molecules (red) compared with corresponding isotype controls (gray). Journal of Allergy and Clinical Immunology 2011 127, 487-494.e3DOI: (10.1016/j.jaci.2010.12.003) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Expression of surface molecules on mDCs Expression of surface molecules on mDCs. Histograms display the expression of surface molecules (red) compared with corresponding isotype controls (gray). Journal of Allergy and Clinical Immunology 2011 127, 487-494.e3DOI: (10.1016/j.jaci.2010.12.003) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions