Paula K. Shireman, MD, Walter J. McCarthy, MD, William H

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Presentation transcript:

Elevated levels of plasminogen-activator inhibitor type 1 in atherosclerotic aorta Paula K. Shireman, MD, Walter J. McCarthy, MD, William H. Pearce, MD, Bruce K. Patterson, MD, Vera P. Shively, MS, Maria Cipollone, BS, Natalia Tamarina, PhD, Elaine N. Verrusio, MS, Hau C. Kwaan, MD Journal of Vascular Surgery Volume 23, Issue 5, Pages 810-818 (May 1996) DOI: 10.1016/S0741-5214(96)70243-4 Copyright © 1996 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 1 PAI-1 levels in aortic tissue-explant supernatants increased over time. PAI-1 levels increased over time in all seven samples. PAI-1 levels in the three aneurysmal specimens (circles) continued to rise over the entire 72-hour period. In contrast, PAI-1 levels in four normal tissue samples (squares) rapidly increased until approximately 24 hours and then remained relatively constant. Journal of Vascular Surgery 1996 23, 810-818DOI: (10.1016/S0741-5214(96)70243-4) Copyright © 1996 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 2 PAI-1 Northern blot analysis. 3.2- and 2.3-kb species of PAI-1 mRNA migrate between the 28S and 18S ribosomal bands. Notice the decreased signal of GAPDH in diseased aorta, indicating uneven loading of RNA (N, normal samples; A, aneurysms; O, occlusive samples; E and H are endothelial cell and Hep G2 tissue culture RNA used as positive control samples, respectively). Journal of Vascular Surgery 1996 23, 810-818DOI: (10.1016/S0741-5214(96)70243-4) Copyright © 1996 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 PAI-1 in-situ hybridization. A, In this aortic arteriole, PAI-1 mRNA localizes to endothelial cells of vasa vasorum (arrow). Original magnification, 40×. B, Sense PAI-1 probes, used as negative control sample, revealed no binding to endothelial cell vasa vasorum. Original magnification, 40×. C, Vascular SMC positive for PAI-1 mRNA. Original magnifacation, 100×. D, Section of aneurysm shows positive lumenal endothelial cells (solid arrow) and scattered positive intimal cells (open arrow). Original magnification, 40× Journal of Vascular Surgery 1996 23, 810-818DOI: (10.1016/S0741-5214(96)70243-4) Copyright © 1996 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 PAI-1 in-situ hybridization. A, In this aortic arteriole, PAI-1 mRNA localizes to endothelial cells of vasa vasorum (arrow). Original magnification, 40×. B, Sense PAI-1 probes, used as negative control sample, revealed no binding to endothelial cell vasa vasorum. Original magnification, 40×. C, Vascular SMC positive for PAI-1 mRNA. Original magnifacation, 100×. D, Section of aneurysm shows positive lumenal endothelial cells (solid arrow) and scattered positive intimal cells (open arrow). Original magnification, 40× Journal of Vascular Surgery 1996 23, 810-818DOI: (10.1016/S0741-5214(96)70243-4) Copyright © 1996 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 PAI-1 in-situ hybridization. A, In this aortic arteriole, PAI-1 mRNA localizes to endothelial cells of vasa vasorum (arrow). Original magnification, 40×. B, Sense PAI-1 probes, used as negative control sample, revealed no binding to endothelial cell vasa vasorum. Original magnification, 40×. C, Vascular SMC positive for PAI-1 mRNA. Original magnifacation, 100×. D, Section of aneurysm shows positive lumenal endothelial cells (solid arrow) and scattered positive intimal cells (open arrow). Original magnification, 40× Journal of Vascular Surgery 1996 23, 810-818DOI: (10.1016/S0741-5214(96)70243-4) Copyright © 1996 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions

Fig. 3 PAI-1 in-situ hybridization. A, In this aortic arteriole, PAI-1 mRNA localizes to endothelial cells of vasa vasorum (arrow). Original magnification, 40×. B, Sense PAI-1 probes, used as negative control sample, revealed no binding to endothelial cell vasa vasorum. Original magnification, 40×. C, Vascular SMC positive for PAI-1 mRNA. Original magnifacation, 100×. D, Section of aneurysm shows positive lumenal endothelial cells (solid arrow) and scattered positive intimal cells (open arrow). Original magnification, 40× Journal of Vascular Surgery 1996 23, 810-818DOI: (10.1016/S0741-5214(96)70243-4) Copyright © 1996 Society for Vascular Surgery and the North American Chapter, International Society for Cardiovascular Surgery Terms and Conditions