Volume 88, Issue 5, Pages L30-L32 (May 2005)

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Volume 88, Issue 5, Pages L30-L32 (May 2005) Differential Labeling of Myosin V Heads with Quantum Dots Allows Direct Visualization of Hand-Over-Hand Processivity  David M. Warshaw, Guy G. Kennedy, Steven S. Work, Elena B. Krementsova, Samantha Beck, Kathleen M. Trybus  Biophysical Journal  Volume 88, Issue 5, Pages L30-L32 (May 2005) DOI: 10.1529/biophysj.105.061903 Copyright © 2005 The Biophysical Society Terms and Conditions

Figure 1 Myosin V processive run with heads labeled with different colored Qdots. Green and red open circles are the Qdot565 and Qdot655 positions, respectively, determined by Gaussian fits. Solid lines are the average Qdot positions between steps with the onset of steps determined by eye. Upper left are averaged Qdot images for steps labeled A–D, with red and green images offset by 12 pixels in y for clarity. The yellow lines connect Qdot centers emphasizing alternating relative head positions. Green arrow identifies substep. Lower right are histograms of interhead spacing and step size. Biophysical Journal 2005 88, L30-L32DOI: (10.1529/biophysj.105.061903) Copyright © 2005 The Biophysical Society Terms and Conditions

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