Chemogenetic modulation of vlPAG glutamatergic neurons bidirectionally modulates nociceptive behaviors. Chemogenetic modulation of vlPAG glutamatergic.

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Chemogenetic modulation of vlPAG glutamatergic neurons bidirectionally modulates nociceptive behaviors. Chemogenetic modulation of vlPAG glutamatergic neurons bidirectionally modulates nociceptive behaviors. A, Illustration showing the strategy for viral targeting of AAV5-hSyn-DIO-hM3Dq–mCherry, AAV5-hSyn-DIO-hM4Di–mCherry, and AAV5-hSyn-DIO-EGFP bilaterally injected into the vlPAG of Vglut2 Cre mice. B, C, Representative images of a coronal sections containing vlPAG showing restricted viral expression following microinjection of the AAV5-hSyn-DIO-hM3Dq–mCherry and AAV5-hSyn-DIO-hM4Di–mCherry into the vlPAG of Vglut2 Cre mice. E, H, CNO (2 mg/kg, i.p.) administration resulted in a significant increase in PWLs but not on PWTs in Vglut2::hM3Dq mice. F, I, CNO (2 mg/kg, i.p.) administration resulted in a significant decrease in PWLs and PWTs in Vglut2::hM4Di mice. D, G, CNO had no significant effect on PWLs or PWTs in Vglut2 Cre mice expressing the control EGFP construct compared with baseline PWLs and PWTs before CNO administration. All values are mean ± SEM, Student’s t test; ∗p < 0.05. Scale bars, 25 μm. Vijay K. Samineni et al. eNeuro 2017;4:ENEURO.0129-16.2017 ©2017 by Society for Neuroscience