Apc deletion within the renal epithelium leads to rapid deletion of recombined cells and an upregulation of senescent markers.A and B. Kidneys imaged on.

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Apc deletion within the renal epithelium leads to rapid deletion of recombined cells and an upregulation of senescent markers.A and B. Kidneys imaged on OV100 microscope. Apc deletion within the renal epithelium leads to rapid deletion of recombined cells and an upregulation of senescent markers.A and B. Kidneys imaged on OV100 microscope. A. Mice at P3 and 6 months imaged for RFP. Note kidneys from AhCre mice without the Rosa26 tdRFP reporter (labelled Neg) show little RFP positivity, whilst AhCre Rosa26 tdRFP mice (labelled RFP) on figure show marked RFP positivity. B. Mice imaged at 2 months (2 mo), top panel and 6 months (6 mo) bottom panels for GFP. Top panel (left kidney), note strong positivity for GFP in AhCre+Z/EG+Apc+/+ (labelled GFP). Middle kidney is from AhCre−Z/EG+Apcfl/fl (labelled Neg) shows only autoflourescence. Right kidney shows no detectable GFP positivity in AhCre+Z/EG+Apcfl/fl (labelled Apc) kidney at 2 months. In these mice recombination can only been seen via staining for GFP by IHC (Supporting Information 1D–F) as single cells are below the detection of the OV100. Bottom panel shows at 6 months a small number of positive foci within AhCre+Z/EG+Apcfl/fl kidneys, suggesting that Apc deficient cells are being deleted. C. β‐catenin IHC showing that there is only a small number of β‐catenin nuclear positive cells within the kidneys of AhCre+Apcfl/fl mice. D. Bar graph showing percentage of AhCre+Apcfl/fl mice with single nuclear β‐catenin cells across kidney, premalignant lesions and renal carcinoma at 2, 6 and 12 months of age. At least ten mice were aged to each timepoint. E. β‐catenin IHC showing nuclear localization in small renal lesions. F–H. IHC performed on AhCre+Apcfl/fl kidneys, showing scattered cells and premalignant lesions staining for p21 (F and G) and p16 (H and I). J. Co‐immunofluorescence (IF) for β‐catenin (red) and p21 (green) showing high expression of both within premalignant lesions from AhCre+Apcfl/fl mice. K and L IHC for p21 (K) and p16 (L) in renal carcinoma from AhCre+Apcfl/fl mice, showing markedly reduced levels compared to premalignant lesions. In all cases scale bars represent 20 µm. Alicia M. Cole et al. EMBO Mol Med. 2010;2:472-486 © as stated in the article, figure or figure legend