Detection of IgG Autoantibodies in the Sera of Patients with Bullous and Gestational Pemphigoid: ELISA Studies Utilizing a Baculovirus-Encoded Form of.

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Presentation transcript:

Detection of IgG Autoantibodies in the Sera of Patients with Bullous and Gestational Pemphigoid: ELISA Studies Utilizing a Baculovirus-Encoded Form of Bullous Pemphigoid Antigen 2 Claudia Haase, Lioba Büdinger, Luca Borradori, Carole Yee, Hans F. Merk, Kim Yancey, Michael Hertl Journal of Investigative Dermatology Volume 110, Issue 3, Pages 282-286 (March 1998) DOI: 10.1038/sj.jid.5602955 Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Scatter blot of reactivities of sera from patients with BP, PG, PV/PF, and NHS in the ELISA with BV13 protein (BPAG2). Immobilized BV13 protein (ECD of BPAG2) was incubated with sera (diluted at 1:20) from adult patients with BP, PG, PV/PF, and NHS. Binding of serum antibodies to immobilized BV13 was visualized by incubation with nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate after incubation with a second, AP- conjugated goat anti-human IgG (1:5000) and was expressed as OD at 405 nm. By the Wilcoxon test, differences in the BPAG2 reactivities were highly significant (p < 0.0001) between sera from BP/PG patients and control sera (NHS and PV/PF sera). The cut-off of the ELISA was set at an ODbv13-wt value of 0.11 that was equal to the mean value of the negative control sera + 1 SD. This cut-off is indicated by the spaced line. Journal of Investigative Dermatology 1998 110, 282-286DOI: (10.1038/sj.jid.5602955) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions