High relative deoxyribonucleic acid content of trophectoderm biopsy adversely affects pregnancy outcomes Shelby A. Neal, M.D., Jason M. Franasiak, M.D.,

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High relative deoxyribonucleic acid content of trophectoderm biopsy adversely affects pregnancy outcomes Shelby A. Neal, M.D., Jason M. Franasiak, M.D., Eric J. Forman, M.D., H.C.L.D., Marie D. Werner, M.D., Scott J. Morin, M.D., Xin Tao, M.S., Nathan R. Treff, Ph.D., Richard T. Scott, M.D., H.C.L.D. Fertility and Sterility Volume 107, Issue 3, Pages 731-736.e1 (March 2017) DOI: 10.1016/j.fertnstert.2016.11.013 Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Method for determination of biopsy DNA content as a surrogate for biopsy size. (A) Quantitative PCR-based mean threshold cycles (CT) from a 96-plex reaction were analyzed on samples with known numbers of cells (1, 2, 3, 4, 5, 10, 15, and 20) from a cultured cell line. (B) A standard curve was established, based on the cell number and average CT value. This curve was used to estimate the relative DNA content of each biopsy. Fertility and Sterility 2017 107, 731-736.e1DOI: (10.1016/j.fertnstert.2016.11.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Pregnancy outcomes across quartiles of trophectoderm biopsy DNA content. The biopsy quartile with highest DNA content (Q4) demonstrated poorer outcomes across all measured outcomes. Fertility and Sterility 2017 107, 731-736.e1DOI: (10.1016/j.fertnstert.2016.11.013) Copyright © 2016 American Society for Reproductive Medicine Terms and Conditions