The Microscope Ms. Duncan Biology 1

Properties of Microscopes Device used to magnify images of structures too small to see with the naked eye Magnification: increases the size of the image Resolution: shows clarity and detail of image Types of Microscopes: Light Microscope & Electron Microscope

Light Microscope Magnifies using visible light rays Simple microscopes: use one lens Compound Light microscopes: use several lenses

Light Microscope Photo: Courtesy of www.2spi.com

Sample Images Photos: courtesy of www.microimaging.ca

Parts of the Compound Microscope Ocular Lens: magnifies image at eyepiece (10X) Eyepiece: where you look into the microscope Objective Lens: the magnifying lens High Power: 40X Medium Power: 10X Low Power: 4X

Parts of the Compound Microscope Nosepiece: turns objectives Stage: where slide sits upon Diaphragm: controls amount of light allowed in view Fine/Coarse Adjustment: adjusts sharpness of image Arm: holds eyepiece and allows you to carry Illuminator: source of light

Using the Microscope Total Magnification: Magnification of objective lens x magnification of eyepiece Example: The eyepiece will always be 10X. If you are using the low power objective (10X) then your total magnification is ???? 10X x 10X = 100X

Using the Microscope Focusing Where to start How

Microscope Development Hans and Zacharis Jannsen: constructed the first microscope Anton van Leeuwenhoek: “Father of Microscopy” *first person to see tiny living organisms living in a drop of water First to study: Romans & Egyptians in 100 AD

Jannsen Compliments of “The Gemmary.” www.gemmary.com

Leeuwenhoek Photos: courtesy of www.ucmp.berkeley.edu

Hooke Photos: courtesy of www.ucmp.berkeley.edu

Electron Microscope Made in Europe during the 1950s, use beams of electrons instead of light. Allows for more precise images

Electron Microscope Two types TEM: Transmission Electron Microscope Shines a beam of electrons thru thin specimen SEM: Scanning Electron Microscope Run pencil-like beam of electrons back and forth across surface of specimen Produce realistic and dramatic 3D images

TEM: transmission electron microscope Shines a beam of electrons thru thin specimen Photo: courtesy of www.bnl.gov

Sample Images Electron Diffraction from a diamond Diseased kidney tissue Gold particle Photos: Courtesy of www.fei.com

SEM: Scanning Electron Microscope Run pencil-like beam of electrons back and forth across surface of specimen Produce realistic and dramatic 3D images

Sample Images Clam Gills Kosher Salt Black Widow Claw