Endothelial cell activation of the smooth muscle cell phosphoinositide 3-kinase/Akt pathway promotes differentiation David J. Brown, MD, Eva M. Rzucidlo,

Slides:

Advertisements

Similar presentations

Volume 68, Issue 4, Pages (October 2005)

Advertisements

Chung S. Lim, MRCS, PhD, Serafim Kiriakidis, PhD, Ewa M

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Therapeutic delivery of hydrogen sulfide for salvage of ischemic skeletal muscle after the onset of critical ischemia Peter W. Henderson, MD, Natalia.

Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure Oscar.

Chung S. Lim, MRCS, PhD, Serafim Kiriakidis, PhD, Ewa M

Beatrice M. Filippi, Clair S. Yang, Christine Tang, Tony K.T. Lam

Regulation of vascular smooth muscle cell differentiation

Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats

Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway Hidenori.

Xue Ma, MD, Jeffrey D. Pearce, MD, David B. Wilson, MD, William P

Folic acid inhibits homocysteine-induced proliferation of human arterial smooth muscle cells Brennan J. Carmody, MD, Subodh Arora, MD, Ricardo Avena,

Hyperphosphatemia induces protective autophagy in endothelial cells through the inhibition of Akt/mTOR signaling Yu-Juei Hsu, MD, PhD, Shih-Che Hsu,

Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α Alan Dardik,

Bertrand Poussier, MD, Alfredo C

Domain-dependent action of urokinase on smooth muscle cell responses

Urokinase-induced smooth muscle cell responses require distinct signaling pathways: A role for the epidermal growth factor receptor Suzanne M. Nicholl,

Angiogenic effects of stromal cell-derived factor-1 (SDF-1/CXCL12) variants in vitro and the in vivo expressions of CXCL12 variants and CXCR4 in human.

Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells Paul DiMuzio, MD, Thomas Tulenko, PhD Journal.

Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the protein expression of inducible nitric oxide.

The cyclolignan picropodophyllin attenuates intimal hyperplasia after rat carotid balloon injury by blocking insulin-like growth factor-1 receptor signaling

Hyperphosphatemia induces protective autophagy in endothelial cells through the inhibition of Akt/mTOR signaling Yu-Juei Hsu, MD, PhD, Shih-Che Hsu,

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Vascular smooth muscle cell peroxisome proliferator-activated receptor-γ deletion promotes abdominal aortic aneurysms Milton Hamblin, PhD, Lin Chang,

Association of smooth muscle cell phenotypes with extracellular matrix disorders in thoracic aortic dissection Lixin Wang, MD, PhD, Jing Zhang, MD, PhD,

Transforming growth factor-β increases vascular smooth muscle cell proliferation through the Smad3 and extracellular signal-regulated kinase mitogen-activated.

M.A. Greene, R.F. Loeser Osteoarthritis and Cartilage

Cell migration in response to the amino-terminal fragment of urokinase requires epidermal growth factor receptor activation through an ADAM-mediated mechanism

The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro Carol J. Sawmiller, MD, Richard J. Powell, MD,

Foxc2 overexpression enhances benefit of endothelial progenitor cells for inhibiting neointimal formation by promoting CXCR4-dependent homing Dujuan.

Adenovirus-mediated intra-arterial delivery of cellular repressor of E1A-stimulated genes inhibits neointima formation in rabbits after balloon injury

Wai-ki Yiu, MBBS, MRCS, Stephen W. K. Cheng, MS, FRCS, Bauer E

Volume 65, Issue 4, Pages (April 2004)

Mechanical stretch induces podocyte hypertrophy in vitro1

Tetrandrine prevents monocrotaline-induced pulmonary arterial hypertension in rats through regulation of the protein expression of inducible nitric oxide.

Daniel J. Wong, MD, Daniel Y. Lu, MD, Clinton D

Sphingosine-1-phosphate–induced oxygen free radical generation in smooth muscle cell migration requires Gα12/13 protein-mediated phospholipase C activation

Overexpression of transforming growth factor–β1 correlates with increased synthesis of nitric oxide synthase in varicose veins Theresa Jacob, PhD, Anil.

Ambient pressure upregulates nitric oxide synthase in a phosphorylated-extracellular regulated kinase– and protein kinase C–dependent manner Angela G.

Smooth muscle cells cultured from human saphenous vein exhibit increased proliferation, invasion, and mitogen-activated protein kinase activation in vitro.

Fibronectin promotes VEGF-induced CD34+ cell differentiation into endothelial cells Errol S Wijelath, PhD, Salman Rahman, PhD, Jacqueline Murray, PhD,

IGF-II-Mediated COX-2 Gene Expression in Human Keratinocytes Through Extracellular Signal-Regulated Kinase Pathway Hye Jung Kim, Tae-Yoon Kim Journal.

Desmuslin gene knockdown causes altered expression of phenotype markers and differentiation of saphenous vein smooth muscle cells Ying Xiao, PhD, Zhibin.

Identification of a homocysteine receptor in the peripheral endothelium and its role in proliferation Hongjiang Chen, MD, PhD, Ryan Fitzgerald, BS, Aliza.

IL-13 dampens human airway epithelial innate immunity through induction of IL-1 receptor–associated kinase M Qun Wu, MD, PhD, Di Jiang, BS, Sean Smith,

IGF-1 Induces SREBP-1 Expression and Lipogenesis in SEB-1 Sebocytes via Activation of the Phosphoinositide 3-Kinase/Akt Pathway Terry M. Smith, Kathryn.

Volume 68, Issue 4, Pages (October 2005)

Sphingosine-1-phosphate–induced smooth muscle cell migration involves the mammalian target of rapamycin William J. Tanski, MD, Suzanne M. Nicholl, PhD,

Gene delivery to in situ veins: Differential effects of adenovirus and adeno-associated viral vectors Mohammad H. Eslami, MD, Sidhu P. Gangadharan, MD,

Navin K. Kapur, MD, Ce Bian, MD, Edward Lin, MD, Clayton B

Cellular stress inhibits vascular smooth muscle relaxation

The effects of doxycycline and micronized purified flavonoid fraction on human vein wall remodeling are not hypoxia-inducible factor pathway-dependent

Nitric oxide prevents p21 degradation with the ubiquitin-proteasome pathway in vascular smooth muscle cells Melina R. Kibbe, MD, Suhua Nie, MD, Dai-Wu.

Gabriele Di Luozzo, MD, Jaya Bhargava, PhD, Richard J. Powell, MD

Upregulation of Tenascin-C Expression by IL-13 in Human Dermal Fibroblasts via the Phosphoinositide 3-kinase/Akt and the Protein Kinase C Signaling Pathways

Protein kinase C-δ regulates migration and proliferation of vascular smooth muscle cells through the extracellular signal-regulated kinase 1/2 Bo Liu,

Contribution of Src-FAK signaling to the induction of connective tissue growth factor in renal fibroblasts A. Graness, I. Cicha, M. Goppelt-Struebe

Chi-Hyun Park, Youngji Moon, Chung Min Shin, Jin Ho Chung

Carotid plaque instability and ischemic symptoms are linked to immaturity of microvessels within plaques Benjamin J. Dunmore, PhD, Mark J. McCarthy,

In vitro differences between smooth muscle cells derived from varicose veins and normal veins Ying Xiao, PhD, Zhibin Huang, MD, Henghui Yin, PhD, Ying.

Green Tea Polyphenol Epigallocatechin-3-Gallate Suppresses Collagen Production and Proliferation in Keloid Fibroblasts via Inhibition of the STAT3-Signaling.

Long-term results of combined common femoral endarterectomy and iliac stenting/stent grafting for occlusive disease Robert W. Chang, MD, Philip P. Goodney,

The effect of growth factors, cytokines, and extracellular matrix proteins on fibronectin production in human vascular smooth muscle cells Terry L. Kaiura,

Smad3 and Extracellular Signal-Regulated Kinase 1/2 Coordinately Mediate Transforming Growth Factor-β-Induced Expression of Connective Tissue Growth Factor.

1α,25-Dihydroxyvitamin D3 Stimulates Activator Protein 1 DNA-Binding Activity by a Phosphatidylinositol 3-Kinase/Ras/MEK/Extracellular Signal Regulated.

IL-13 dampens human airway epithelial innate immunity through induction of IL-1 receptor–associated kinase M Qun Wu, MD, PhD, Di Jiang, BS, Sean Smith,

Interleukin-1β inhibits PDGF-BB−induced migration by cooperating with PDGF-BB to induce cyclooxygenase-2 expression in baboon aortic smooth muscle cells

Dustin M. Bermudez, MD, Junwang Xu, MD, Benjamin J

Class 1 phosphatidylinositol 3-kinase (PI3K) p85 subunit dominant negative adenovirus regulates Pax2 and GAPDH abundance in NRK-52E cells. Class 1 phosphatidylinositol.

IGF-1 regulation of type II collagen and MMP-13 expression in rat endplate chondrocytes via distinct signaling pathways M. Zhang, Ph.D., Q. Zhou, M.D.,

Presentation transcript:

Endothelial cell activation of the smooth muscle cell phosphoinositide 3-kinase/Akt pathway promotes differentiation David J. Brown, MD, Eva M. Rzucidlo, MD, Bethany L. Merenick, BS, Robert J. Wagner, BS, Kathleen A. Martin, PhD, Richard J. Powell, MD Journal of Vascular Surgery Volume 41, Issue 3, Pages 509-516 (March 2005) DOI: 10.1016/j.jvs.2004.12.024 Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 1 Co-culture with endothelial cells induces a differentiated smooth muscle cell (SMC) morphology. A, Photomicrograph (40× magnification) comparing cell morphology of SMCs cultured alone (SMC) or SMCs co-cultured with endothelial cells (CC) for 48 hours (see Materials and Methods). B, Two-dimensional cell area as measured by planimetry. Results are mean ± SEM. *P < .05 vs SMC alone. Journal of Vascular Surgery 2005 41, 509-516DOI: (10.1016/j.jvs.2004.12.024) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 2 Co-culture induces biochemical markers of smooth muscle cell (SMC) differentiation and activates SMC Akt. A, SMCs were cultured alone or co-cultured opposite endothelial cells (CC) for 48 hours and were analyzed by Western blotting with antibodies recognizing smooth muscle 2-myosin heavy chain (SM2-MHC), phospho-serine 473 (P-Ser473) Akt, total Akt, and phospho-serine 9 glycogen synthase kinase 3β (P-GSK-3β). B, Cells were cultured as above in A and SMCs analyzed by Western blotting with antibodies recognizing smoothelin, P-Ser473 Akt, total Akt, P-Ser9 GSK-3β, p27kip, and total extracellular signal-regulated kinase (ERK1/2) (loading control). C, Corresponding densitometry data for SM2 (*P < 0.05), P-Ser473 Akt (** P < .008), and total Akt from three replicate experiments. Results are mean ± SEM. Journal of Vascular Surgery 2005 41, 509-516DOI: (10.1016/j.jvs.2004.12.024) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 3 Co-culture-conditioned media induces smooth muscle cell (SMC) differentiation and activates SMC Akt. A, Conditioned media from SMC and endothelial cell 24-hour co-culture was added at time 0 to SMC cultured alone. SMCs were analyzed at the subsequent time points indicated by Western blotting as in Fig 2. (P-Ser473, phospho-serine 473) B, SMCs were treated with conditioned media as in A and analyzed at the subsequent time points indicated by Western blotting for the indicated antibodies (ERK1/2 [extracellular signal-regulated kinase] loading control) SM2-MHC, smooth muscle 2-myosin heavy chain). Journal of Vascular Surgery 2005 41, 509-516DOI: (10.1016/j.jvs.2004.12.024) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 4 Overexpression of constitutively active Akt induces smooth muscle cell (SMC) differentiation. SMC were cultured alone and infected with adenovirus encoding green fluorescent protein (GFP) only (Ctrl), constitutively active hemagglutinin (HA)-tagged myristoylated Akt (MyrAkt), or were uninfected (Uninf) overnight. Cells were washed, re-fed, and assayed by Western blotting 24 hours later with antibodies recognizing smooth muscle 2-myosin heavy chain (SM2-MHC), the HA-epitope tag (HA-myrAkt), phospho-serine 9 glycogen synthase kinase 3β (GSK-3β), and total extracellular signal-regulated kinase (ERK1/2) (loading control). Journal of Vascular Surgery 2005 41, 509-516DOI: (10.1016/j.jvs.2004.12.024) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 5 Wortmannin prevents co-culture-induced differentiation. Smooth muscle cells (SMCs) were cultured alone or co-cultured opposite endothelial cells for 48 hours, and then 100 nM wortmannin or vehicle control was added to the SMCs for the 48-hour duration. A, SMC area as determined by planimetry. Black bar, SMC cultured alone with vehicle; black striped bar, SMC in co-culture with vehicle (P < 0.05 relative to all others); gray bar, SMC cultured alone with wortmannin; gray striped bar, SMC in co-culture with wortmannin. Data are mean ± SEM. B, SMC were treated as above in A and analyzed by Western blotting as in Fig 2 (SM2-MHC, smooth muscle 2-myosin heavy chain). Journal of Vascular Surgery 2005 41, 509-516DOI: (10.1016/j.jvs.2004.12.024) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

Fig 6 Dominant-negative Akt (dnAkt) inhibits co-culture-induced differentiation. Smooth muscle cells (SMCs) were infected with dnAkt or control (green fluorescent protein [GFP]) adenoviruses, or uninfected overnight, washed, trypsinized, and replated opposite confluent endothelial cells in co-culture (CC) for 48 hours. Two-dimensional cell area as measured by planimetry is shown. Solid bars, SMC alone; stippled bars, SMC in co-culture. Data are mean ± SEM. *P < .05 for uninfected-CC or control virus-CC vs all other groups. Journal of Vascular Surgery 2005 41, 509-516DOI: (10.1016/j.jvs.2004.12.024) Copyright © 2005 The Society for Vascular Surgery Terms and Conditions