Do Now How many ul (microliters) are in one liter?

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Presentation transcript:

Do Now How many ul (microliters) are in one liter?

Objectives 1. SWBAT practice using the micropipette. 2. SWBAT measure out 20 ul, 10 ul, 5 ul, and 2ul.

Micropipetting Three types: Very expensive instrument for measuring *** A set of 3 (p20, p200, p1000) can range from $750- $1,000***** Measures very small volumes: microliters (ul) _________ microliters = 1 liter Three types: P-1000 __________ - __________ P-200 __________ - __________ P-20 __________ - __________ https://www.youtube.com/watch?v=uEy_NGDfo_8

Micropipette What is the volume of this pipette? 2 20 ul

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Using the Micropipette Determine the volume to be pipette. Rotate the dial to the appropriate setting. NEVER GO ABOVE THE MAXIMUM VOLUME THAT IS MADE FOR THE MICROPIPETTE Use a clean pipette tip from box. Gently push micropipette onto tip. USE PROPER STERILE TECHNIQUE To draw up solution – depress plunger to first stop; release plunger and draw up solution; place tip at bottom of tube; depress plunger to second stop. Eject the used tip over and into the waste basket on table.

Holding the Pipette WSSP Lab Video